DSpace Coleção:http://repositorio.ufgd.edu.br/jspui/handle/prefix/3962023-11-24T16:32:38Z2023-11-24T16:32:38ZAvaliação da toxicidade e das atividades anti-inflamatória e analgésica do extrato etanólico de Gomphrena celosioides Mart. (Amaranthaceae) em roedoresMacorini, Luis Fernando Benitezhttp://repositorio.ufgd.edu.br/jspui/handle/prefix/49542023-09-14T07:04:38Z2021-05-07T00:00:00ZTítulo: Avaliação da toxicidade e das atividades anti-inflamatória e analgésica do extrato etanólico de Gomphrena celosioides Mart. (Amaranthaceae) em roedores; Anti-inflammatory, analgesic and toxicological activity of the ethanol extract Gomphrena celosioides Mart. (Amaranthaceae); Actividad antiinflamatoria, analgésica y toxicológica del extracto etanólico Gomphrena celosioides Mart. (Amaranthaceae)
Autor(es): Macorini, Luis Fernando Benitez
Primeiro Orientador: Arena, Arielle Cristina
Abstract: Introduction: Gomphrena celosioides, is popularly known in Brazil as “Perpétua Brava” and used for the treatment of several diseases: liver, dermatological, dysmenorrhea, infections, pain, among others. Chemical study of the ethanolic extract of the leaves showed the presence of malic acid, caffeic acid, ferulic acid, catechin, vanillic acid, irisone B, dimethoxyflavone, caffeyl-glucose, the biological activities of the plant have already been described as anti-inflammatory, diuretic, antimicrobial, gastroprotection, antioxidant and immunomodulators. In this context, the objective of the research was to evaluate the anti-inflammatory, anti arthritic, analgesic and toxicological potential of the ethanolic extract of Gomphrena celosioides (EEGC). Materials and Method: Paw edema, mechanical hyperalgesia and cold allodine were induced in male Swiss mice with carrageenan after oral treatment of EEGC at doses of 300, 700 and 1000 mg/kg, control and 1 mg/kg dexamethasone. The female Swiss mouse pleurisy model was induced after administration of 1 mL of intrapleural carrageenan and the animals treated with EEGC at doses of 300, 700 and 1000 mg/kg, control and dexamethasone (1 mg/kg). For arthritis induction, C57Bl6 mice were induced with intra-articular zymosan and after 30 minutes treated with EEGC 300 mg / kg, control and dexamethasone 1 (mg/kg). For the evaluation of peritonitis, male Swiss mice were divided into a naive group, control, EEGC 300 mg/kg and dexamethasone (1 mg/kg). 30 minutes after treatment, peritonitis was induced by zymosan. Leukocyte rolling and adhesion in mesenteric endothelium was evaluated after carrageenan induction and treatment with EEGC 300 mg/kg and Indomethacin (5 mg/kg). The persistent model for anti-inflammatory and analgesic evaluation was performed with Freund's Complete Adjuvant (CFA) in C57BL6 mice treated with EEGC 100 mg/kg, saline control and dexamethasone 1 mg/kg for 22 days. The toxicological evaluation was determined by an acute model using Wistar rats following OECD 2008a guideline and subacute was performed with Swiss mice evaluated for 28 days, evaluating hypocratic, behavioral, histological, biochemical and hematological parameters. Results: EEGC showed a reduction in Paw Edema in all doses in the 3 hours evaluated, it was still possible to observe a reduction in mechanical hyperalgesia in the third hour in all evaluated doses and a reduction in cold allodine with 3 hours in the dose of 700 mg / kg and with 4 hours in 700 and 1000 mg/kg in a model induced by carrageenan. After induction of pleurisy, it was possible to observe a reduction in leukocyte migration at doses of 700 and 1000 mg/kg, but there was no reduction in protein leakage in the pleural cavity. A reduction in leukocyte migration and mechanical hyperalgesia was observed in a model of arthritis induced by Zymosan after treatment with EEGC 300 mg/kg. EEGC also reduced leukocyte migration in Zymosan-induced peritonitis at a dose of 300 mg/kg, but there was no difference in the nitric oxide (NO) dosage. After assessing the reduction of leukocytes in the peritoneal cavity, the leukocyte rolling and adhesion in mesenteric endothelium was evaluated and a reduction in both rolling and adhesion was observed after induction with carrageenan and treatment with 300 mg/kg EEGC. In a persistent CFA model, animals treated with EEGC 100 mg/kg reduced paw edema on the 22nd day, cold allodynia on 6, 11 and 16 days and reduced mechanical hyperalgesia on all evaluated days. The acute toxicological analysis after administration of 2000 mg/kg in Wistar rats, there was no change in the parameters evaluated as well as no changes in the parameters evaluated for subacute toxicity at doses of 75, 150 and 300 mg / kg. Conclusion: EEGC demonstrated anti-inflammatory, antiarthritic and analgesic potential in different models of acute and persistent induction, with decreased bearing and leukocyte endothelial adhesion. Still, it did not present toxicity in an acute and sub-acute evaluation model.
Editor: Universidade Federal da Grande Dourados
Tipo: Tese2021-05-07T00:00:00ZAvaliação do potencial farmacológico do extrato aquoso das folhas de Acrocomia aculeata (Jacq.) Lodd ex. MartAlfredo, Tamaeh Monteirohttp://repositorio.ufgd.edu.br/jspui/handle/prefix/49392023-09-14T07:00:22Z2021-10-29T00:00:00ZTítulo: Avaliação do potencial farmacológico do extrato aquoso das folhas de Acrocomia aculeata (Jacq.) Lodd ex. Mart
Autor(es): Alfredo, Tamaeh Monteiro
Primeiro Orientador: Souza, Kely de Picoli
Abstract: The increase in life expectancy of the world population is directly related to the incidence of chronic diseases such as neurodegenerative and cardiovascular diseases, rheumatoid arthritis, diabetes, cancer, among others. The impact of the treatment of these diseases and the maintenance of health represents a high economic and social costs. Oxidative stress is a key factor associated with the etiology and progression of these diseases. Thus, the development of therapeutic alternatives is of fundamental importance, especially with low cost, easy access, and, above all, with relevant pharmacological potential. Medicinal plants, especially those with antioxidant potential, are an important source of natural compounds that can be used as raw materials in the development of new therapies. Acrocomia aculeata is a palm native of Brazil, whose leaves are rich in phenolic and flavonoid compounds, with a proven antioxidant effects. Thus, our objective was to evaluate the effect of the aqueous extract of A. aculeata leaves (EAAa) in two conditions related to oxidative stress, the complications associated with diabetes and the cardiotoxicity induced by chemotherapy with doxorubicin (Dox). For the investigation of the effects associated with diabetes complications, normoglycemic Wistar rats and non-obese type 2 diabetic rats, Goto-Kakizaki (GK), were treated daily with 200 mg.kg-1 EA-Aa for 30 days and evaluated in relation to the anthropometric and biochemical parameters. After treatment, the animals were anesthetized and euthanized. Proteins associated with hyperglycemia and oxidative stress were evaluated in target organs, in 3T3-L1 pre-adipocytes, and in dermal microvascular cells (HMVec-D) induced by H2O2 to redox imbalance. The results showed that EA-Aa reduced fasting glycemia and triglycerides of diabetic rats, by increasing the levels of proteins such as GLUT-4, PPARγ and AMPK and reduced oxidative stress in 3T3- L1 pre-adipocytes and HMVec-D cells, in addition to promoting higher levels of proteins related to stress resistance, SIRT1 and NRF2. Regarding the effects of EA-Aa in association with Dox, a chemotherapeutic agent known for its high cardiotoxicity caused by oxidative stress, in vitro experimental models were used: normal cells (PBMC peripheral blood cells and H9c2 cardiomyoblasts) and tumor cells (K562 erythroleukemics) and breast cancer MCF-7), and in vivo, C57Bl/6 mice, treated with EA-Aa, with or without Dox. EA-Aa showed no toxicity in normal cells in vitro and in the acute oral toxicity test in vivo. Regarding the effect of EA-Aa associated with Dox, there was a reduction in oxidative stress induced in erythrocytes, in H9c2 cells and in cardiotoxicity in C57Bl 6 mice, in addition to potentiating the cytotoxic effect of Dox in tumor cells. Together, our results show the antioxidant protection of EA-Aa in diabetic complications and in those resulting from treatment with Dox in various tissues, as well as its mechanisms of action. Thus, the data presented here support new studies based on the pharmacological potential of EA-Aa and its phytochemicals for the development of therapeutic strategies for the treatment of both conditions.
Editor: Universidade Federal da Grande Dourados
Tipo: Tese2021-10-29T00:00:00ZAvaliação da segurança do extrato aquoso obtido da casca de Plinia cauliflora (Mart.) Kausel: da toxicidade aguda a disrupção endócrinaPalozi, Rhanany Alan Calloihttp://repositorio.ufgd.edu.br/jspui/handle/prefix/49382023-09-14T07:03:37Z2021-09-28T00:00:00ZTítulo: Avaliação da segurança do extrato aquoso obtido da casca de Plinia cauliflora (Mart.) Kausel: da toxicidade aguda a disrupção endócrina
Autor(es): Palozi, Rhanany Alan Calloi
Primeiro Orientador: Gasparotto Junior, Arquimedes
Abstract: Popularly known as “jaboticaba” or “jabuticaba”, a Plinia cauliflora (Mart.) Kausel (Myrtaceae) and a fruit appreciated both for fresh consumption and for the production of jellies, juices and liqueurs. Its most widespread traditional use involves the treatment of diarrhea, which takes advantage of all parts of the plant, including the fruit peels. The study aimed to elucidate the possible risks of administering an ethanol-soluble fraction obtained from the infusion of P. cauliflora fruit peels (SEIPC). For this, a series of experiments were carried out to evaluate a possible toxicity of SEIPC, in which it was administered orally, acutely and repeatedly for 28 days. Also evaluated the possible endocrine disrupting and genotoxic effects on eukaryotic cells through comet and micronucleus assays. SEIPC was produced and chemically prepared by LC-DAD-MS. Acute toxicity and how behavioral and physiological changes were evaluated in the modified Irwin test. After 28 days of oral treatment, parameters such as respiratory rate, arterial blood gases, electrocardiography, respiratory and heart rates, blood pressure, hematological, biochemical and histopathological analyses were obtained. The comet assay, the micronucleus test, the uterotrophic test, the Hershberger bioassay, and the AMES test were performed using external links. Phenolic acids such as gallic acid and ellagic acid and derivatives, flavonols and anthocyanidins, as well as citric acid were identified from the ethanolic supernatant of the infusion of Plinia cauliflora by LC-DAD-MS. After both acute and prolonged treatments, the present study found that SEIPC did not cause significant changes in several body systems, including electrical activity, body temperature, respiratory rate and blood pressure. Furthermore, no changes in biochemical, hematological or blood gas parameters were observed. It was also possible to verify that SEIPC did not cause any disturbances in the endocrine system or mutagenic, cytotoxic or genotoxic effects. These findings support the safe use of P. cauliflora.
Editor: Universidade Federal da Grande Dourados
Tipo: Tese2021-09-28T00:00:00ZAnálise antinociceptiva, antiartrítica e de toxicidade de extratos das folhas de Doliocarpus dentatus (Aubl.) StandlBranquinho, Lidiane Schultzhttp://repositorio.ufgd.edu.br/jspui/handle/prefix/49292023-09-14T07:00:08Z2020-12-14T00:00:00ZTítulo: Análise antinociceptiva, antiartrítica e de toxicidade de extratos das folhas de Doliocarpus dentatus (Aubl.) Standl
Autor(es): Branquinho, Lidiane Schultz
Primeiro Orientador: Kassuya, Cândida Aparecida Leite
Abstract: Several experimental models have been developed to study the inflammatory process and the associated pain, hence, these models are important for the discovery of new drugs. Medicinal plants are useful sources for developing new pharmacological therapies since conventional medicines could cause many side effects. Doliocarpus dentatus (Dilleniaceae) is used in folk medicine to relieve pain related to the inflammatory process and the literature reports few evidences of analgesic and anti-inflammatory effects. Thus, the objective of the study was to assess the antinociceptive and antiarthritic and determine the toxic potential of the aqueous (EADd) and ethanolic (EEDd) extracts from leaves of D. dentatus in in vivo experimental models. Compounds were identified in EADd by UHPLC-HRMS (Ultra high-performance liquid chromatography coupled to high resolution mass spectrometry). Male and female Swiss mice and C57BL/6 male mice were used for the tests. The oral dose of 17 mg/kg EADd, calculated according to ethnopharmacological uses and doses of 30, 100 and 300 mg/kg were used to test in formalin and acetic acid-induced models of pain and behavior. EADd (100, 1000 e 2000 mg/kg) was assayed in mice by comet, micronucleus and phagocytosis tests. Doses of 30, 100 and 300 mg/kg of EEDd was tested in formalin and acetic acid-induced models of pain. EEDd (100 and 300 mg/kg) was also tested in the assays of chronic inflammation with complete Freund's adjuvant (CFA) and joint inflammation induced by zymosan. Betulinic acid of 0.3, 3 e 30 mg/kg and EEDd 100 e 300 mg/kg were used in the zimosan-induced peritonitis model. In the cell viability test (MTT), EEDd was tested at 3, 10, 30 and 90 μg/mL. In the acute toxicity test, 500, 1000 and 2000 mg/kg EEDd were administered in a single dose. Doses of 75, 150 and 300 mg/kg EEDd were administered for subacute toxicity for 28 days and behavioral parameters were analyzed. Oral treatments with EADd and EEDd significantly inhibited nociceptive sensitivity in neurogenic and inflammatory phase and decreased formalin-induced cold hypersensitivity and also prevented nociceptive behavior in the model of acetic acid-induced abdominal contortions. In the CFA model, the treatment for 21 days with EEDd significantly inhibited mechanical hyperalgesia, edema and cold response. In the same way, EEDd prevented joint inflammation (mechanical hyperalgesia, knee edema and leukocyte migration) induced by zymosan. In the peritonitis model, betulinic acid (compound present in 0.23% of the EEDd) and EEDd significantly inhibited zymosan-induced leukocyte infiltration, however, did not produce significant effects on the concentration of nitrite. In addition, in vitro treatment did not induce leukocyte cytotoxicity. In the acute toxicity test with EEDd, LD50 above 2000 mg/kg was observed. Oral treatment for 28 days with EDD did not changed in the main organs, hematological and biochemical parameters and also did not induce depression or anxiety. Genetic assays in mice treated with EADd revealed no significant differences in genotoxic cell damage and did not alter phagocytic activity or peripheral leukocyte count, indicating that EADd has no genotoxic potential. Analyzing the results, we can conclude that could act as an antinociceptive agent that does not present genotoxicity and that EEDd exhibited antiarthritic potential, with no signs of toxicity and symptoms of depression or anxiety in mice. The compounds identified at the species such as betulinic acid, trigonelline and isoquercetin may be related to the activities observed in the extracts. This study contributes to justify, in part, the popular use of D. dentatus in pain management, ensuring its safe use.
Editor: Universidade Federal da Grande Dourados
Tipo: Tese2020-12-14T00:00:00Z