DSpace Coleção:http://repositorio.ufgd.edu.br/jspui/handle/prefix/5002023-12-01T04:22:28Z2023-12-01T04:22:28ZSíntese e estudo da atividade antioxidante e antitumoral de compostos N,S - acetais utilizando biocatalisadores enzimáticosAlbuquerque, Tábata Branquinho dehttp://repositorio.ufgd.edu.br/jspui/handle/prefix/50322023-09-14T05:47:04Z2021-04-05T00:00:00ZTítulo: Síntese e estudo da atividade antioxidante e antitumoral de compostos N,S - acetais utilizando biocatalisadores enzimáticos
Autor(es): Albuquerque, Tábata Branquinho de
Primeiro Orientador: Domingues, Nelson Luís de Campos
Abstract: Biocatalysis research has grown exponentially in recent decades due to discoveries related to the synthesis of many compounds, mainly those with biological properties, which are beneficial to society. In addition, environmental concerns have led many research groups around the world, and even industries, to describe new synthetic protocols that are not harmful to the environmental and to considere the byproducts formed by reactions. Thus, the use of catalysts allows reactions more quickly using low-cost reagents and avoids the waste of solvents, which have a great impact on the environmental factor. In this sense, the use of enzymes has excellent catalytic characteristics such as high efficiency and biodegradation (instead of heavy metals); and in some cases, chemical transformations using enzymes occur in the aqueous medium, avoiding the use of polluting solvents. Many examples of enzymatic reactions have been described in the literature, and in view of the need to synthesize compounds in a more “green” way, interest arises in establishing a synthetic methodology for N,S-acetals, which are commonly found in a wide range of biological products with medical and biochemical applications. However, as far as we know, no other procedure has been described for the synthesis of N,S-acetals using biological catalysts, such as enzymes, other than the work reported by our own research group. In addition, the researchers are looking for a methodological innovation in reactors, which are greener and easier to handle. Thus, in this work, we proposed to use enzymes in the synthesis of some N,S-acetals under mild reaction conditions, besides to presenting an enzymatic reactor project based on the continuous flow chemistry applied in the synthesis of N,S-acetal. In this study, we proved that the LPP enzyme immobilization was effective and satisfactory and it was possible to reuse it in other cycles. The methods established using both free and immobilized enzymes proved to be tolerant for both thiol and amidosulfone, getting high yields in a short reaction time. In addition, N,S-acetals were evaluated to determine their antioxidant property and antitumor potential against the SK-MEL-19, SK-MEL-28, SK-MEL-103 and B16F10-Nex2 melanoma cell lines, and gratifying results were obtained with these assays.
Editor: Universidade Federal da Grande Dourados
Tipo: Tese2021-04-05T00:00:00ZAvaliação das alterações fisiológicas em sementes, plântulas e folíolos de Ingá laurina (Fabaceae) induzidas por nanopartículas de óxido de cobre, ferro e zincoGraciano, Daniela Espanguerhttp://repositorio.ufgd.edu.br/jspui/handle/prefix/50312023-09-14T05:48:28Z2020-04-06T00:00:00ZTítulo: Avaliação das alterações fisiológicas em sementes, plântulas e folíolos de Ingá laurina (Fabaceae) induzidas por nanopartículas de óxido de cobre, ferro e zinco
Autor(es): Graciano, Daniela Espanguer
Primeiro Orientador: Caires, Anderson Rodrigues Lima
Abstract: The technological advancement of nanotechnology in modern society presents us with the challenge of generating data and information that support the normative evolution and legislation to regulate the applications of nanoparticles (NPs) used in industry, as well as the use, storage and disposal of NPs in the environment. The present study aimed to evaluate and characterize the physiological functioning of Inga laurina seeds when exposed to copper oxide nanoparticles (CuONPs), iron (Fe2O3NPs) and zinc (ZnONPs); For this purpose, the germination and root growth indices were determined when submitted to different concentrations of NPs and the changes in the photosynthetic capacity of the seeds were evaluated by chlorophyll a fluorescence image analyses during the germination period and in young plants, 30 days after exposure. The results obtained for CuONPs showed a reduction in flourescence intensity over time for treatment 50 mgL -1 when compared to the Control group (H2O, 0 mgL -1). In other cases, an increase was observed for the other concentrations (100, 200 and 300 mgL -1). Changes in fluorescence intensity were a consequence of the reduction (50 mgL -1) or increase (100, 200 and 300 mgL -1) in chlorophyll content promoted by CuONPs. It was also observed that CuONPs induced oscillations in seedling temperature over the seven days in all treatments. The results showed that the CuONPs did not impact the germination process, presenting germination rates of 100% for all treatments. However, root growth was impacted by CuONPs, in which a reduction in root enlogation was observed for all concentrations. The results also demonstrated that the functioning of the photosynthetic apparatus and the temperature of the seeds/seedlings/leaflets were impacted by Fe2O3NPs and ZnONPs in a similar way to that observed for CuONPs, in which there was a gradual increase in photochemical efficiency due to the increase in the concentration of nanoparticles Germination rates were 100% for all treatments for both nanoparticles. However, unlike CuONPs, Fe2O3NPs and ZnONPs did not impact root development. In summary, the present study shows for the first time that, depending on the concentration, CuONPs, Fe2O3NPs and ZnONPs could positively or negatively impact the physiological status of plant Inga laurina in its initial stage of development, mainly altering the efficiency of the operation of photosystem II.; El avance tecnológico de la nanotecnología en la sociedad moderna nos plantea el reto de generar datos e información que apoyen la evolución normativa y la legislación para regular las aplicaciones de las nanopartículas (NP) utilizadas en la industria, así como el uso, almacenamiento y eliminación de NP en el medio ambiente. El presente estudio tuvo como objetivo evaluar y caracterizar el funcionamiento fisiológico de las semillas de Inga laurina cuando se exponen a nanopartículas de óxido de cobre (CuONPs), hierro (Fe2O3NPs) y zinc (ZnONPs). Para ello, se determinaron los índices de germinación y crecimiento radicular cuando se sometieron a diferentes concentraciones de NP y los cambios en la capacidad fotosintética de las semillas evaluadas por clorofila y análisis de imagen de fluorescencia durante el período de germinación y en plantas jóvenes, 30 días después de la exposición. Los resultados obtenidos para CuONPs mostraron una reducción en la intensidad de la fluorescencia a lo largo del tiempo para el tratamiento 50 mgL -1 en comparación con el grupo Control (H2O, 0 mgL - ). En otros casos, se observó un aumento para las otras concentraciones (100, 200 y 300 mgL -1) Los cambios en la intensidad de la fluorescencia fueron consecuencia de la reducción (50 mgL -1) o aumento (100, 200 y 300 mgL -1) en el contenido de clorofila promovido por Los CuONPs. También se observó que los CuONPs indujeron oscilaciones en la temperatura de las plántulas durante los días pares en todos los tratamientos. Los resultados mostraron que los CuONPs no impactaron en el proceso de germinación, presentando tasas de germinación del 100% para todos los tratamientos. Sin embargo, el crecimiento de la raíz se vio afectado por los CuONPs, en los que se observó una reducción en la enología radicular para todas las concentraciones. Los resultados también demostraron que el funcionamiento del aparatus fotosintético y la temperatura de las semillas/plántulas/folíolos se vieron afectados por Fe2O3NPs, ZnONPs de manera similar a la observada para CuONPs, en la que hubo un aumento gradual de la eficiencia fotoquímica debido al aumento en la concentración de nanopartículas. Las tasas de germinación fueron del 100% para todos los tratamientos tanto para nanopartículas. Sin embargo, a diferencia de los CuONPs, Fe2O3NPs y ZnONPs no afectaron el desarrollo radicular. En resumen, el presente estudio muestra por primera vez que, dependiendo de la concentración, CuONPs, Fe2O3NPs y ZnONPs podrían impactar positiva o negativamente en el estado fisiológico de la planta Inga laurina en su etapa inicial de desarrollo, alterando principalmente la eficiencia del funcionamiento del fotosistema II.
Editor: Universidade Federal da Grande Dourados
Tipo: Tese2020-04-06T00:00:00ZAvaliação do potencial biotecnológico de leveduras industriaisNascimento, Valkirea Matoshttp://repositorio.ufgd.edu.br/jspui/handle/prefix/45962023-09-14T06:50:50Z2021-03-23T00:00:00ZTítulo: Avaliação do potencial biotecnológico de leveduras industriais
Autor(es): Nascimento, Valkirea Matos
Primeiro Orientador: Fonseca, Gustavo Graciano
Abstract: Sacharomyces cerevisiae CAT-1 stands out as the strain with the highest fermentation capacity proven to date. However, S. cerevisiae FT858 was recently presented as a potential alcoholic fermenter. Thus, the aim of this work was to evaluate the physiology of S. cerevisiae FT858 in terms of substrate consumption, formation of extracellular metabolites, cell growth and invertase activity in different carbon sources to compare it with S. cerevisiae CAT-1. S. cerevisiae FT858 presented kinetic growth parameters very close to those obtained with S. cerevisiae CAT-1 under similar culture conditions
(aerobiosis, temperature, agitation, substrate and concentration). However, there was a 10% increase in sucrose yield into ethanol obtained with S. cerevisiae FT858 when compared to S. cerevisiae CAT-1 at 37 °C. The concentration of the substrates directly interfered in the production of invertase for both S. cerevisiae FT858 and CAT-1. The enzymatic expression underwent strong regulation through the concentration of glucose in the culture medium. However, only S. cerevisiae CAT-1 showed a constitutive behavior for the enzyme invertase. In a second study, a total of 14 yeast strains, including S. cerevisiae CAT-1, were also evaluated in terms of kinetic parameters and compared. In general, yeasts of the genus Kluyveromyces showed greater respiratory potential while yeasts of the genus Saccharomyces had a greater fermentative capacity. Among other results, it is noteworthy that Kluyveromyces marxianus NRRLy 2415 was the largest producer of biomass (23.17 ± 0.000 C-X), while S. cerevisiae BG-1 was the smallest producer (3.05 ± 0.000 C-X).
Editor: Universidade Federal da Grande Dourados
Tipo: Tese2021-03-23T00:00:00ZBioconversão da polpa de bocaiuva verde por Pleurotus ostreatus para obtenção de bolo low carbGiunco, Aline Janainahttp://repositorio.ufgd.edu.br/jspui/handle/prefix/44962023-09-14T06:43:31Z2021-02-12T00:00:00ZTítulo: Bioconversão da polpa de bocaiuva verde por Pleurotus ostreatus para obtenção de bolo low carb
Autor(es): Giunco, Aline Janaina
Primeiro Orientador: Fonseca, Gustavo Graciano
Abstract: The bocaiuva is a cerrado palm, widely distributed in almost all of Brazil, being abundant in Mato Grosso do Sul and Mato Grosso. The pulp of the ripe fruit has important nutritional, sensory and functional characteristics for health. However, it is a fruit little used in human food, mainly in the stage of green maturation and there is little information about the nutritional and functional properties of this fruit in its different species, mainly of Acrocomia totai. Therefore, the objective of the study was the bioconversion of the green bocaiuva pulp by Pleurotus ostreatus to obtain a low carb cake, aiming to obtain a food product with nutritional, functional and sensorially accepted by consumers. In the evaluation of the growth and the proximal composition of the edible mushroom Pleurotus ostreatus in green pulp substrates with different nitrogen sources (8 treatments) showed that the addition of ammonium nitrate as a nitrogen source in the T3 treatment potentiated the bioconversion of carbohydrates from green bocaiúva pulp of Pleurotus ostreatus in proteins, fibers and lipids of valuable nutritional potential, which is promising for the development of new food products with added nutraceutical value. In the development and evaluation of low-carbohydrate cakes produced from the pulp of green bocaiúva enriched with pleurotus ostreatus, it showed that the green bocaiúva flour enriched with P. ostreatus can be used for the development of low carb cake with enhanced nutritional components and acceptable sensory characteristics. This information can support studies on the nutritional properties of the green pulp of bocaiúva enriched with pleurotus ostreatus, as well as enabling the biotechnological application and large-scale production of Acrocomia species.
Editor: Universidade Federal da Grande Dourados
Tipo: Tese2021-02-12T00:00:00Z