Desenvolvimento e caracterização de filmes multilayer a partir de polihidroxialcanoatos, hidrocoloides e soro de leite

Abstract

It is of utmost importance to use sustainable alternatives for the reduction of waste in the environment. We studied the physiology Burkholderia sacchari LFM 101 in kinetic terms, using glucose as the carbon source and whey as co-substrate at 30 to 35 °C. Through trials with B. sacchari, it was observed that the cultures using glucose as the sole carbon source at 35 °C presented the best results achieved kinetic parameters compared to other assays. The cultures performed with glucose and whey obtained values lower than crops using glucose as sole carbon source. From these data we assessed the growth profile of lactose as co-substrate, given that mainly lactose and whey component, and that it could be indicated that suppressing the glucose uptake in cultures containing cheese whey. However, lactose has remained constant over the cultures and did not affect the glucose consumption. These results showed that B. sacchari metabolizes better glucose, presenting a higher yield of PHB at 35 °C. This condition was chosen for the production of PHAs on a larger scale for later develops the biofilms. As the whey was not as efficient as a co-substrate for PHA production and is a residue rich in nutrients, it was used to prepare the same multilayer film, evaluating the mechanical, physical, and thermal barriers. For preparation of multilayer films were prepared one phylogenetic solution containing gelatin and whey, plasticized by glycerol. From the results it is noted that films multilayer had values above the standard of pure PHA film and films containing whey and highest concentration of gelatin showed better physical, mechanical, thermal and structural properties.