Análise toxicogenética e atividade antimicobacteriana in vitro do composto flavona

Abstract

Flavonoids are polyphenolic compounds found in medicinal plants, scientific studies show that some flavonoids have anti-inflammatory activity, antioxidant and anticarcinogenic. The flavone (2-phenyl-4H-1-benzopyran-4-one) is a flavonoid with heart stimulating activity, antibacterial, antiallergic and inhibition or stimulation of enzymes. Thus, this study evaluated the anti-mycobacterial effects of flavone in vitro on H37Rv ATCC 27294. strain was also performed acute toxicity tests, genotoxicity and mutagenicity with flavone in vivo. The minimum inhibitory concentration (MIC) flavone was measured at concentrations of 0.98 to 250 mg / mL. Experimental models of acute toxicity and genotoxicity have been developed in female mice orally is flavone administered by gavage in test of acute toxicity at doses of 175, 560, 1792 and 2000 mg / kg-1 and 175 560, 1792 mg / Kg1 in genotoxicity and mutagenicity assays. Hippocratic signs were observed as well as biochemical analyzes were performed, hematological, genotoxic (comet assay, micronucleus) and mutagenic (phagocytosis). The flavone presented antimycobacterial activity MIC = 31.25 mm / ml. The data obtained was found to increase in macrophage recruitment into the splenic tissue was an average 55% phagocytosis of cells compared to the negative control in 37% phagocytosed cells, these data demonstrate that the flavone could play immunostimulatory activity. In vivo tests were not observed signs of toxicity and no changes in biochemical, hematological, genotoxic and mutagenic parameters. Macroscopic examination of organs such as kidneys, liver and lungs showed no significant difference between groups. Thus, this study demonstrates the flavone has vitro antimycobacterial activity (efficacy against Mycobacterium tuberculosis). In addition, the flavone is not toxic in the models made in this work. These results together lead to the demonstration of efficacy against mycobacteria flavone and safety of a compound present in nature.