Estabelecimento de modelo experimental para indução de Doença Renal Crônica (DRC) através de nefrectomia 5/6 (Nx) em ratos Wistar

Abstract

Chronic Kidney Disease is a major public health problem presenting mortality a range of over 50% among kidney patients. The most common animal model used in preclinical studies in kidney disease is represented by 5/6 nephrectomy, allowing the comparison with chronic renal disease in humans. Objectives: establish the surgical model of 5/6 nephrectomy for chronic kidney disease induction. Methods: Eight male gender rats were used in this experiment, which were weighing approximately 450 g and submitted to 5/6 nephrectomy. Blood samples were taken 24 hours before the experiment and 48 hours after for determination of serum creatinine. Results: It was observed ischemia in the posterior and antero-inferior part of the left kidney of the operated animals, which suggests a successful execution of artery obstruction and experiment, although only 12.5% survival of the animals survived. Conclusion: The experimental model is achievable, provided that specific care with all the steps involved in the process due to the high impact on the animal, but which presents with viable alternative for the experiments about the disease. However measures to control death should be better suited as a way to ensure the success of the procedure for a minimum period of changes in blood tests.