Bactérias heterotróficas isoladas de águas subterrâneas

Abstract

Water is an indispensable resource for the survival of all living species. Despite the regulations being more restrict and the advances in water treatment technology, in the past 25 years, diseases of water propagation have emerged as one of the main Public Health issue, reducing population life expectancy. The purpose of this study was the isolation and identification of heterotrophic bacteria presented at underground water, and evaluation of the susceptibility profile to the antibiotic's. The search for these bacteria happened among 66 underground wells situated in two different cities in Mato Grosso do Sul state, both with less than 30 thousand inhabitants. 56 bacteria were isolated, being 30,45% gram positive and 69,64% Gram negatives. Micrococcus luteus was the most present bacteria among the isolated, being in 19,14% of samples. Serratia marcesces was presented in 17,02% of samples and Acinetobacter haemolyticus in 8,51%. The Gram positive did not show resistance to any of the 16 antibiotics tested, while from the Gram negative isolated, 28,57% showed resistance to two or more antibiotics. From those, 62,5% was resistent to ampicillin, 50% to ampicillin/sulbactam, cefuroxime and piperacilline/tazobactam, 37,5% to cefoxitin, ceftriaxone and collistin, and 25% to ceftadizime. The isolation of the species associated to clinic infections and the high resistance profile of negative Gram microorganisms from that environment show a need, first for the implementation of a sturdier program of hygienic and sanitary control of this water, then for the propagation of the knowledge about the quality of underground water to all the community.