Técnicas de conservação do sistema cardiovascular em espécies de diferentes grupos de vertebrados

Abstract

The use of anatomical specimens preserved favors educational processes and reduce the euthanasia of animals in periodic anatomical studies scholars. The correct fixation and preservation of anatomical specimens are essential to prevent contamination, respiratory, neurological and immune related in people who maintain direct contact with them, as teachers, technicians and students. In this context, the objective of this study was to evaluate the efficacy of different preservation techniques of the cardiovascular system of different vertebrate groups. For this, we used hearts of fish (Salminus maxillosus), birds (Gallus gallus) and mammals (Sus domesticus). The specimens were subjected to the techniques of fixation and glicerinação. Glicerinação The technique consisted of three steps: dehydration, bleaching and fixing-drying. The parameters evaluated, immediately after withdrawal and after the procedures were: external morphology, color, consistency and flexibility. The result of analysis of each parameter was categorized into 1) Efficient: to describe the pieces that remained above 75% similarity on the trait in relation to the recently withdrawn, (2) Intermediate: for the parts that remained between 50 to 75% Like the trait for sin newly withdrawal, (3) Inefficient: For pieces that have lost more than 50% of the trait in relation to the recently withdrawn. Our results show that the technique of formaldehyde was Intermediate in appearance and color Inefficient for external morphology, consistency and flexibility. As for technique glicerinação, the results were presented for Intermediate external morphology, Efficient and Inefficient for color for consistency and flexibility. In conclusion, the technique glicerinação had the best set of results for the conservation of parts and thus is considered the most appropriate study for preservation of hearts in different vertebrates used for academic activities.