Desenvolvimento de procedimentos analíticos para determinação de clotrimazol e diclofenaco utilizando espectrofotometria na região ultravioleta

Abstract

Analytical procedures employing molecular spectroscopy are based on the measurement of radiation absorbed or generated by the substance of interest. Spectroscopic methods are widely used for qualitative and quantitative determination of different analytes in different samples. The molecular absorption spectrophotometry in the ultraviolet-visible (UV-vis) is an analytical method recognized for its advantages such as speed, low operating costs and produce reliable results and simple interpretation. In this work the optimization of analytical methods for the determination of clotrimazole and sodium diclofenac in pharmaceutical samples. After the optimized solvent afforded an analytical curve with a linear range of concentration ranging between clotrimazole 2.95 x10-5 to 5.90 × 10-4 mol L- 1. A detection limit 5.33x10-7 mol L-1 and quantitation limit of 1.00 × 10-5 mol L-1 were obtained. The recoveries of clotrimazole in the studied samples ranged from 91.8 to 100%. For the determination of sodium diclofenac, the procedure presented an analytical curve with a linear range of concentration between 3.9×10-6 to 3.9×10-5 mol L-1. A detection limit of 1.60 x10-7 mol L-1 was obtained. A limit of quantification of 5.33x10-7 mol L- 1 was obtained in optimized conditions . The recoveries of diclofenac in the samples were higher than 115 %. The treatment of the spectra (derivative spectrophotometry) allowed the use of the procedure , enabling us diclofenac recoveries between 97.2 to 106% .