Enzimas amilolíticas na ensilagem de milho reidratado armazenada por diferentes períodos

Abstract

With the greater use of technological tools in animal production systems, corn silage has become the most prevalent and most prevalent food in Brazil. However, despite the characteristics that make maize a food used in animal nutrition and the main ingredient used in the manufacture of silage, most of the hybrids grown in Brazil have high vitreous, which limits their digestibility. For this reason, the addition of enzyme compounds to the animal diet has been used more frequently. This study aimed to evaluate amylolytic enzyme and storage time effects on fermentative losses, aerobic stability, nutritional value, fermentative and microbiological profile and enzymatic activity in rehydrated ground corn silage. One hundred and twenty experimental silos were prepared, divided in three treatments: 1CON (without enzyme); 2- α-AMI, (α-amylase, Kerazyme 3035, 300 mL t-1 of fresh matter); 3 - GLU, (glucoamylase, Kerazyme 4560, 300 mL t-1 of fresh matter). All silos were also inoculated with L. plantarum (4.0×1010 ufc g-1) and P. acidipropionici (2.6 ×1010 ufc g-1) added at 4 g t-1 of hydrated ground corn. The silos were opened every 30 days during the period of 240 days of storage. Data were submitted to analysis of variance using the PROC MIXED of SAS 9.3. Long periods of storage and addition of amylases negatively influenced fermentation losses, aerobic stability, reduced DM, starch, CNF, IVDMD and increased CP, EE, EB and lignin. There was a reduction of pH, acetate and butyrate and increase in ethanol and lactate concentration comparing the silos plus enzymes to the control. The inclusion of enzymes did not increase the population of lactic acid bacteria and did not stabilize the population of fungi and yeasts over time. Thus, over time the modifications caused by the inclusion of the enzymes have altered more negatively the evaluated aspects of the food, therefore more studies are necessary to understand the functioning of these compounds inside the silo and thus to be able to define models that take more use effective.