http://repositorio.ufgd.edu.br/jspui/handle/prefix/854 2026-08-14T09:24:29Z 2026-08-14T09:24:29Z Cabanha, Fernando da Silva http://repositorio.ufgd.edu.br/jspui/handle/prefix/6875 2026-08-12T05:10:44Z 2026-04-13T00:00:00Z

Título: Avaliação ambiental da aquicultura em tanques-rede em um assentamento rural: aplicação de ferramentas de sustentabilidade integrada Autor(es): Cabanha, Fernando da Silva Primeiro Orientador: Albuquerque, Daniele Menezes Abstract: Aquaculture represents an important productive activity for food supply and economic development. However, its expansion requires careful attention to environmental impacts and sustainability. In this context, this study analyzed Brazilian scientific production on aquaculture from an environmental perspective, aiming to identify patterns and trends in how research has addressed environmental impacts, monitoring, and assessment of aquatic organism farming practices. A cienciometric analysis was conducted using the Scopus database, applying the search strategy (“aquaculture” OR “fish farm*” OR “pisciculture”) AND (“environmental impact” OR “environmental assessment” OR “environmental monitoring”). After applying filters and manual validation, 203 articles published between 1996 and 2024 were selected. Analyses performed with the support of Excel, VOSviewer, and Biblioshiny indicated a significant increase in scientific output from 2010 onwards, highlighting the role of public institutions and international collaborations. The main research themes included water quality, heavy metals, sustainability, life cycle assessment, and microplastics. The results indicate that Brazilian research on aquaculture and the environment has consolidated as a multidimensional and interdisciplinary field, although gaps remain, particularly regarding small-scale aquaculture, continental systems, and socio-environmental approaches. Editor: Universidade Federal da Grande Dourados Tipo: Dissertação

2026-04-13T00:00:00Z Andrade , Bruna Aparecida Mortari de http://repositorio.ufgd.edu.br/jspui/handle/prefix/6874 2026-08-12T05:10:58Z 2026-02-26T00:00:00Z

Título: Avaliação da viabilidade produtiva de meio alternativo à base de Lemna minor para o cultivo de Chlorella sp Autor(es): Andrade , Bruna Aparecida Mortari de Primeiro Orientador: Paz, Marcelo Fossa da Abstract: Currently, microalgae represent one of the most promising sources for generating bio-inputs, but the greatest difficulty faced is the high cost of culture media. Therefore, given the scarcity of studies on new formulations, the present work aims to evaluate the behavior of the microalga of the genus Chlorella in a medium enriched with the extract of the macrophyte Lemna minor, which is easily found in large quantities in the natural environment, to quantify the amount of protein generated by the microalga in an alternative medium, and to verify the effectiveness of cryopreservation of the species through the maintenance of its viability. The water sample was obtained from the Itá stream, in the Barra do Itá settlement, in Bela Vista – MS. Isolation was performed using the “streak plating” method, in which a sample of the obtained water was subjected to streaking using a platinum loop on a Petri dish containing a solid medium composed of agar (1%) and NPK agricultural fertilizer (10-10-10 1.0 g.L-1). After plating, the plates were kept on a bench under a 16-hour photoperiod until the first isolated colonies grew. To prepare the extract, 2.5% w/v of dried and crushed L. minor was added to distilled water, which was kept boiling for 15 minutes. Tests showed that the medium enriched with 1% and 2% Lemna minor was more nutritious for Chlorella sp. compared to the adapted BBM medium. Macronutrient analyses of the 2.5% Lemna minor extract show that this macrophyte has a high nutritional content, largely due to its ability to absorb large quantities of nutrients present in the culture medium. However, further academic research is needed on the use of this macrophyte as a nutrient source for other microorganisms and on other potential nutrient suppliers for new alternative media formulations. The study also demonstrated that the cryopreservation of Chlorella sp. At -80°C using 10% glycerol as a cryoprotectant, it is viable for short to medium term storage (up to 42 days), maintaining cell viability and recovery capacity after thawing. Editor: Universidade Federal da Grande Dourados Tipo: Dissertação

2026-02-26T00:00:00Z Silva, Ediléia Amancio http://repositorio.ufgd.edu.br/jspui/handle/prefix/6730 2025-11-30T04:17:35Z 2019-05-14T00:00:00Z

Título: Avaliação de parâmetros físicos-químicos e fitotoxicidade das águas do Córrego Paragem Autor(es): Silva, Ediléia Amancio Primeiro Orientador: Vieira, Heberth Juliano Abstract: The stream Paragem is born south of the city of Dourados and crosses the urban and agricultural areas of the municipality. Its environment presents intense occupation, being subject to the most diverse environmental impacts. However, another complicating is the occupations and irregular ocuppancy that occur to a great extent of the stream. The present study had as objective to analyze the water quality at four points of the stream stops, with emphasis on physical-chemical analysis and phytotoxicity through germination and elongation Lactuca sativa. Four sampling points were selected, being two controls that are groundwater and osmosis water, in which water samples collected in April, May, and June of 2018. Water analysis consisted of pH, electrical conductivity, Redox Potential, turbidity, total solids, total dissolved solids, ammonia, as well as Principal Component Analysis and hierarchical analysis of the data. The phytotoxicity of the water samples was evaluated using the length of root and hypocotyl of the Lactuca sativa seedlings. Box tests followed by Tukey tests were used for the analysis of variance of root and hypocotyl lengths obtained at each sampling point. The sites studied Nascente, Palmeiras, Aguiar, and Frei Antônio were the electrical conductivity was above the limits of the current legislation in Brazil. The Frei Antônio presented the worst water quality with electrical conductivity and total dissolved solids higher than the current Brazilian legislation. It was concluded that physical-chemical analysis, together phytotoxicity of samples from stream water stopping using Lactuca sativa, was a valid indicator of environmental conditions and are useful tools to assess and compare the toxicity of stream surface water. Editor: Universidade Federal da Grande Dourados Tipo: Dissertação

2019-05-14T00:00:00Z Marques, Otavio Gonçalves Benites http://repositorio.ufgd.edu.br/jspui/handle/prefix/6675 2025-10-08T05:11:19Z 2021-05-02T00:00:00Z

Título: Estudo fisiológico de Saccharomyces cerevisiae CAT-1 em resting cell após cultivo em diferentes condições Autor(es): Marques, Otavio Gonçalves Benites Primeiro Orientador: Paz, Marcelo Fossa da Abstract: The use of microorganisms to obtain products and services is an ancient practice that has been used empirically since the beginning of the society. Studies on cell metabolism are of paramount importance for a better biochemical understanding and for the establishment of appropriate cultivation conditions for obtaining bioproducts of interest through “cell factories”. The aim of this work was to evaluate the physiology of resting cells of the yeast Saccharomyces cerevisiae CAT-1 after carbon and nitrogen pulses. M9 mineral medium with adaptations and a nitrogen source limitation were utilized to establish the resting cells state. Kinetic studies to substrate consumption and secondary metabolites production were performed in microcultures using a microplate reader and posteriorly were evaluated in HPLC chromatography. The use of a lower concentration of nitrogen source (0,25 mM de NH4Cl) allowed the establishment of the best resting condition (0.5 optical density curve) to carry out the pulses. After these, growth was restored at the sucrose 0,1% condition with increase of biomass reaching absorbance values of 0.764, however there was no increase in the ethanol production. A second limiting condition was observed in the treatment with the addition of the nitrogen source in the absence of the carbon source, which may have affected cell growth and even the mechanism of glycolysis. In addition to variations in substrate consumption and metabolite production, invertase activity was also measured during the resting period and after the pulses. Results indicated presence of enzymatic activity (2,11 U mL-1) at the exact moment of the sucrose addition in the medium, explaining the constitutive nature of the enzyme. Although the aim of this work had been reached, there is a necessity of additional studies to better understanding the metabolic behavior in response to these disturbance. Editor: Universidade Federal da Grande Dourados Tipo: Dissertação

2021-05-02T00:00:00Z