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Avaliação de parâmetros físicos-químicos e fitotoxicidade das águas do Córrego Paragem

2025-11-30T04:17:35Z

Título: Avaliação de parâmetros físicos-químicos e fitotoxicidade das águas do Córrego Paragem Autor(es): Silva, Ediléia Amancio Primeiro Orientador: Vieira, Heberth Juliano Abstract: The stream Paragem is born south of the city of Dourados and crosses the urban and agricultural areas of the municipality. Its environment presents intense occupation, being subject to the most diverse environmental impacts. However, another complicating is the occupations and irregular ocuppancy that occur to a great extent of the stream. The present study had as objective to analyze the water quality at four points of the stream stops, with emphasis on physical-chemical analysis and phytotoxicity through germination and elongation Lactuca sativa. Four sampling points were selected, being two controls that are groundwater and osmosis water, in which water samples collected in April, May, and June of 2018. Water analysis consisted of pH, electrical conductivity, Redox Potential, turbidity, total solids, total dissolved solids, ammonia, as well as Principal Component Analysis and hierarchical analysis of the data. The phytotoxicity of the water samples was evaluated using the length of root and hypocotyl of the Lactuca sativa seedlings. Box tests followed by Tukey tests were used for the analysis of variance of root and hypocotyl lengths obtained at each sampling point. The sites studied Nascente, Palmeiras, Aguiar, and Frei Antônio were the electrical conductivity was above the limits of the current legislation in Brazil. The Frei Antônio presented the worst water quality with electrical conductivity and total dissolved solids higher than the current Brazilian legislation. It was concluded that physical-chemical analysis, together phytotoxicity of samples from stream water stopping using Lactuca sativa, was a valid indicator of environmental conditions and are useful tools to assess and compare the toxicity of stream surface water. Editor: Universidade Federal da Grande Dourados Tipo: Dissertação

Estudo fisiológico de Saccharomyces cerevisiae CAT-1 em resting cell após cultivo em diferentes condições

2025-10-08T05:11:19Z

Título: Estudo fisiológico de Saccharomyces cerevisiae CAT-1 em resting cell após cultivo em diferentes condições Autor(es): Marques, Otavio Gonçalves Benites Primeiro Orientador: Paz, Marcelo Fossa da Abstract: The use of microorganisms to obtain products and services is an ancient practice that has been used empirically since the beginning of the society. Studies on cell metabolism are of paramount importance for a better biochemical understanding and for the establishment of appropriate cultivation conditions for obtaining bioproducts of interest through “cell factories”. The aim of this work was to evaluate the physiology of resting cells of the yeast Saccharomyces cerevisiae CAT-1 after carbon and nitrogen pulses. M9 mineral medium with adaptations and a nitrogen source limitation were utilized to establish the resting cells state. Kinetic studies to substrate consumption and secondary metabolites production were performed in microcultures using a microplate reader and posteriorly were evaluated in HPLC chromatography. The use of a lower concentration of nitrogen source (0,25 mM de NH4Cl) allowed the establishment of the best resting condition (0.5 optical density curve) to carry out the pulses. After these, growth was restored at the sucrose 0,1% condition with increase of biomass reaching absorbance values of 0.764, however there was no increase in the ethanol production. A second limiting condition was observed in the treatment with the addition of the nitrogen source in the absence of the carbon source, which may have affected cell growth and even the mechanism of glycolysis. In addition to variations in substrate consumption and metabolite production, invertase activity was also measured during the resting period and after the pulses. Results indicated presence of enzymatic activity (2,11 U mL-1) at the exact moment of the sucrose addition in the medium, explaining the constitutive nature of the enzyme. Although the aim of this work had been reached, there is a necessity of additional studies to better understanding the metabolic behavior in response to these disturbance. Editor: Universidade Federal da Grande Dourados Tipo: Dissertação

Uso e ocupação do solo, parâmetros limnológicos e Índice de Proteção da Vida Aquática como ferramentas integradas para análise da qualidade da água de nascentes

2025-09-06T05:21:42Z

Título: Uso e ocupação do solo, parâmetros limnológicos e Índice de Proteção da Vida Aquática como ferramentas integradas para análise da qualidade da água de nascentes Autor(es): Silva, Carina Doffinger da Primeiro Orientador: Barufatti, Alexeia Abstract: The intensification of land use and occupation, especially due to the expansion of soybean monoculture, has caused changes in the natural landscape and compromised the quality of water resources, especially in spring areas located in Permanent Preservation Areas (APP). Considering the environmental relevance of the Dourados River Basin (MS), this study evaluated the impacts of land use and occupation on the water quality of three springs called NI, NII and NIII, with different percentages of riparian vegetation cover (>30%, between 10–30% and <10%, respectively). The methodology integrated limnological analyses (physical-chemical parameters and nutrients), remote sensing and the application of the Aquatic Life Protection Index (IVA), conducted between October 2021 and March 2022, during the soybean cultivation cycle. The data were analyzed by nonparametric statistical methods and Principal Component Analysis (PCA). The results indicated that the spring with the lowest vegetation cover (NIII) presented higher concentrations of total nitrogen and total phosphorus, evidencing the influence of intensive agriculture. The PCA revealed a positive correlation between vegetation and IVA in the most protected springs (NI and NII) and a negative correlation between IVA and intensive agriculture in the spring with the least protection (NIII). These findings reinforce the importance of riparian vegetation for maintaining water quality, indicating the need for integrated environmental monitoring strategies that combine geotechnology techniques, limnological analyses and ecological indices such as IVA. Editor: Universidade Federal da Grande Dourados Tipo: Dissertação

Estudo teórico do modelo espectral da viga de Euler-Bernoulli com acoplamento de sensor à base de niobato de potássio e sódio

2025-08-16T05:14:43Z

Título: Estudo teórico do modelo espectral da viga de Euler-Bernoulli com acoplamento de sensor à base de niobato de potássio e sódio Autor(es): Vermieiro, Jeferson Samuel Lobato Primeiro Orientador: Falcão, Evaristo Alexandre Abstract: The present research aims to present the modeling of ceramic sensors based on potassium and sodium niobate (KNN) and the use of the Spectral Element Method in the study of vibrations in structures. In this sense, computational simulations were developed based on the Euler-Bernoulli beam theory, applied to lead-free ceramics, analyzing the simulation results with existing literature with KNN sensors. Editor: Universidade Federal da Grande Dourados Tipo: Dissertação