DSpace Coleção:
http://repositorio.ufgd.edu.br/jspui/handle/prefix/2746
2023-11-23T22:56:36ZEfeito antiadipogênico das folhas de Schinus terebinthifolius Raddi
http://repositorio.ufgd.edu.br/jspui/handle/prefix/4800
Título: Efeito antiadipogênico das folhas de Schinus terebinthifolius Raddi
Autor(es): Orué, Sarah Lam
Primeiro Orientador: Rocha, Paola dos Santos da
Abstract: Obesity is a multifactorial chronic inflammatory disease, characterized by the excessive accumulation of triglycerides in adipocytes, a process called adipogenesis. It is considered one of the risk factors for the development of comorbidities with high rates of morbidity and mortality, such as type 2 diabetes mellitus and cardiovascular diseases. In this sense, substances that reduce the process of adipogenesis are sought, aiming at treatments that improve the quality of life of obese individuals, and prevent the development of associated diseases. Schinus terebinthifolius Raddi (Anacardiaceae), popularly known as red-mastic or pink-pepper is a plant species that has among its constituents, phenolic compounds, which are attributed different biological activities, such as antioxidant, antidiabetic and cardioprotective activities. In this context, the aim of this study was to evaluate the effect of methanolic extract of S. terebinthifolius Raddi leaves (MESL) on viability and adipogenesis in 3T3-F442A cells. For this, 3T3-F442A cells were submitted to cell viability test in MTT and differentiation assay (adipogenesis) with Oil red staining. MESL did not change cell viability at concentrations of 50 and 100 µg/ml within 24h, within 48h the concentration of 50 µg/ml was shown to be safe and at 100 µg/ml EMFS reduced cell viability by 19,24 ± 3,21%. MESL inhibited the accumulation of triglycerides at 25, 50 and 100 µg/ml, acting independently of the concentration, with an average inhibition of 40 ± 2%, similarly to quercetin (42 ± 6%), showing the modulation of the process of adipogenesis. In conclusion, the data suggest that MESL has anti-adipogenic properties, being a potential source for the development of phytopharmaceuticals for the prevention and treatment of obesity and obesity-related morbidities.
Editor: Universidade Federal da Grande Dourados
Tipo: Trabalho de Conclusão de Curso2021-11-26T00:00:00ZPropriedades antioxidante e anti-inflamatória do extrato etanólico do cerume de abelhas sem ferrão Geotrigona sp
http://repositorio.ufgd.edu.br/jspui/handle/prefix/4799
Título: Propriedades antioxidante e anti-inflamatória do extrato etanólico do cerume de abelhas sem ferrão Geotrigona sp
Autor(es): Corrêa, Raíssa Cristina Darroz
Primeiro Orientador: Campos, Jaqueline Ferreira
Abstract: Bee products are natural products commonly used in folk medicine because of their healing properties. Among these is the cerumen, a mixture of the wax integrated by bees, with the propolis they generate from plant exudates and mandibular secretions. The genus Geotrigona includes important pollinators that use cerumen to build nests. There are no scientific reports on the antioxidant and anti-inflammatory capacity of cerumen produced by Geotrigona, which may lead to the prevention of cancer diseases. Therefore, the objective of this is to evaluate the antioxidant and anti inflammatory capacity of the ethanolic extract of cerumen from Geotrigona sp. For this, the cerumen obtained in Quimís, Manabí province, Ecuador was used for the preparation of the EECG, in the proportion 1:4.5 m:v. The quantification of phenolic compounds was performed using the aluminum chloride method and the flavonoids using the Follin-Cicauteau method. To evaluate the antioxidant activity, the free radical scavenging capacity was analyzed using the DPPH (2,2-diphenyl-2-picryl-hydrazyl) and ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-acid) radicals. To evaluate the inhibition of oxidative hemolysis, the EECG (50-125 µg/mL) was incubated with the oxidizing agent AAPH (2,2'-azobis-2-amidinopropane) and the inhibition of lipid peroxidation was evaluated by reduction The anti-inflammatory potential of the extract was evaluated by its ability to inhibit the activity of the enzyme hyaluronidase under hyaluronic acid. As results, phenolic compounds were quantified being 7.08 ± 0.05 mg EAG/g of earwax and flavonoids being 1.42 ± 0.29 mg EQ/g of earwax. EECG inhibited concentration-dependent DPPH and ABTS radicals, with IC50 of 0.97 mg/mL and 0.48 mg/mL, respectively. Furthermore, it inhibited oxidative hemolysis by 45.9% at a concentration of 75 µg/mL. Moreover, it inhibited the lipid peroxidation induced by AAPH by 36% at the concentration of 50 µg/mL after 180 min of incubation and by 41.7 and 28.7% at the concentrations of 50 and 75 µg/mL, respectively, after 240 min. The EECG demonstrated an inhibitory potential on the hyaluronidase enzyme at a concentration of 5 mg/mL of approximately 11.6% compared to the solvent control. Thus, it is highlighted that the EECG has antioxidant and anti-inflammatory activity, demonstrating that natural products such as beekeeping are alternatives for bioprospective studies and the conservation of these species is necessary.
Editor: Universidade Federal da Grande Dourados
Tipo: Trabalho de Conclusão de Curso2021-11-22T00:00:00ZReflexos das atividades antropogênicas: genotoxicidade em peixes no médio Rio Aquidauana, Pantanal Sul
http://repositorio.ufgd.edu.br/jspui/handle/prefix/4798
Título: Reflexos das atividades antropogênicas: genotoxicidade em peixes no médio Rio Aquidauana, Pantanal Sul
Autor(es): Lima, Nathalya Alice de
Primeiro Orientador: Barufatti, Alexeia
Abstract: The Pantanal is the biome with one of the largest wetlands in the world, with great biodiversity, being annually flooded by the Upper Paraguay River Basin, where the Aquidauana River is one of its main tributaries. The Aquidauana River has the function of supplying several municipalities, an economic and tourist source, however, it undergoes disturbances from human activities, such as excessive fishing, riverside pollution and agro-industrial activities. The aim of the study was to evaluate the water quality of the Aquidauana River through physicochemical parameters and to identify possible genotoxic damage in Pantanal fish species. Therefore, the research was conducted in the middle course of the Aquidauana River at six sampling points. With a multiparameter probe, the physicochemical parameters were measured. The fish were collected with waiting nets, their length and weight measured. The species Hypostomus regani, Prochilodus lineatus, Brycon hilarii and Mylossoma duriventre were sampled and identified. For the genotoxicity assay, blood smears were prepared and nuclear changes were counted
under an optical microscope. The physicochemical parameters measured were within the standards allowed by CONAMA legislation 357/2005, or there is no maximum value. The physicochemical parameters are important to identify the first water quality status. In relation to length, weight and feeding habits, B. hilarii had the highest weight and length, followed by the species H. regani, P. lineatus and M. duriventre. Regarding feeding habits, H. regani and
P. lineatus are detritivores and B. hilarii and M. duriventre are omnivores. Nuclear alterations were identified as nuclear invagination, nuclear budding, lobulated nucleus and binucleate cell, where fishes of the species H. regani presented higher proportions of significant nuclear alterations. The feeding habit of H. regani favors contamination, as it feeds on material decanted from the bottom of the river. Species with detritivorous habits showed a higher
proportion of genotoxicity in relation to omnivorous eating habits. In short, the assessment of the water quality of the Aquidauana River through physical-chemical parameters proved to be in compliance with current legislation. However, only physicochemical analyzes do not prove the quality of the Aquidauana River, requiring further tests. Thus, it was possible to verify that
the Aquidauana River has been undergoing environmental disturbances arising from human activities, which may not only affect the organisms that live in the river, but also the populations that consume this water daily.
Editor: Universidade Federal da Grande Dourados
Tipo: Trabalho de Conclusão de Curso2021-10-10T00:00:00ZAnálise temporal do uso do solo por imagens de satélite pré classificadas de algumas bacias hidrográficas do município de Dourados, Mato Grosso do Sul
http://repositorio.ufgd.edu.br/jspui/handle/prefix/4797
Título: Análise temporal do uso do solo por imagens de satélite pré classificadas de algumas bacias hidrográficas do município de Dourados, Mato Grosso do Sul
Autor(es): Morais, Layra Carienne de
Primeiro Orientador: Sciamarelli, Alan
Abstract: The expansion of sugarcane took place in areas previously occupied by livestock and can be explained by the scarcity of land in the main sugarcane producing regions and the availability of land in Mato Grosso do Sul. Studies related to the temporal characterization of the use and occupation of the land are essential for the definition of public policies for territorial planning, allowing an understanding of the dynamics of occupation of territories and how this affects the water resources of a hydrographic basin. This paper presents an analysis of the temporal space of the dynamics of land use and occupation in some hydrographic basins of some rivers in the Cerrado Biome and Atlantic Forest areas in the municipality of Dourados, State of Mato Grosso do Sul, between the years 1985 to 2019 Starting from satellite images pre-classified by the MapBiomas project, the evolution of territorial occupation was analyzed. Analyzing the data, there was a progressive reduction in the areas of native vegetation and the exchange of use of pasture areas for areas destined for sugarcane. The evolution of land use change took place at an accelerated pace, mainly from 2005. As a result of the analysis, in both Biomes, pasture areas lost more than 70% and the class of other temporary crops lost more than 90% of its area in the time range. The forest formation class had an increase of 23% in this period, demonstrating a pale concern for the environment, as these are areas that have not shown investment in preservation, but a decrease in the management of agro-pastoral areas.
Editor: Universidade Federal da Grande Dourados
Tipo: Trabalho de Conclusão de Curso2021-11-26T00:00:00Z