Teor de constituintes, avaliação antioxidante, efeitos antidiabéticos, antiglicação e anticolinesterásica de raízes Cochlospermum regium (Schrank) Pilg. em Ratos Wistar

Abstract

Hyperglycemia is the main consequence of Diabetes Mellitus, which is chronic related to the formation of free radicals, specifically Advanced Glycation End Products (AGES) and inflammatory processes such as nephropathy and neuropathy. Cochlospermum regium (Schrank) Pilg., Bixaceae, is a cerrado plant known for its anti-inflammatory power. The objective of this work was to analyze the contents of the constituents, to evaluate the antioxidant, antiglycation, antidiabetic and anticholinergic effects of the hydrometanolic extract of C. regium roots, through experimental models in vitro and in vivo. The total phenolics, flavonoids, condensed tannin and flavonol were analyzed. The content of the major constituents was evaluated through High Performance Liquid Chromatography (HPLC). The antioxidant activity was investigated through the DPPH, ABTS, β-carotene / linoleic acid and malondialdehyde methods. Inhibition of AGES was examined by a bovine serum albumin system whose glycosylating agent was glucose. Inhibition of disaccharidase was assessed by in vitro and in vivo methods. There was analysis of the inhibition of acetylcholinesterase (Ache) in brain structure. The antidiabetic potential was performed in normoglycemic animals that received glucose overload, in diabetic animals induced with alloxan and in animals that received a hyperglycemic diet. The hydromethanolic extract presented high concentration of phenolic compounds, flavonoids and an antioxidant effect. Liquid chromatography revealed a higher concentration of gallic acid followed by caffeic acid and ellagic acid. There was significantly inhibition of AGES formation. The TOTG of normoglycemic animals demonstrated a significant response at a dose of 100 mg / kg C. regium, accompanied by an increase in hepatic glycogen content. There was also a significant reduction in fasting glucose of alloxan-induced diabetic animals after 7 days of treatment with daily doses of 100 mg / kg. After 14 weeks of hyperglycemia diet, 4 of the treatment with a dose of 100mg / kg, there was a decrease in triglycerides. Thus, the extract of C. regium showed promising antioxidant, antiglycation and antidiabetic effects that may be related to its high phenolic content.