Exercício de força e whey protein durante jejum intermitente em ratos wistar destreinados

Abstract

Intermittent fasting is an alternative strategy that could promote effects similar to continuous dietary restriction. However, inadequate feed of daily protein and fasting can be linked to reduction fat-free mass. Thus, the aim of this study was analyse the acute effect of strength exercise associated with whey protein consumption during acute intermittent fasting (12hs) in intracellular targets of protein synthesis. Male wistar rats (n=48) were divided in rest and exercised in 8 groups. The exercised were submitted to acute strength exercise in a weight- lifting model. Concomintat comsuption of whey protein or water control before 12 hours of intermittent fasting was investigated. The expression of 4EBP1, phospo-4EBP1 and heat shock proteins (HSP70 and HSP90) was analyzed by imunoblotting. Consumption of whey proteins and/or strength exercise before fasting period increases phospho-4EBP1 expression in the same extent in the soleus muscle and overall 4EBP1 was increased with comsuption of whey protein. Fasting induces an increase of phospho-4EBP1 in the diaphragm and was an important effect on muscle HSP70 expression. However, there were no significant changes in the expression of HSP90 in the soleus. The association of protein consumption and and strength exercise before fasting period increases the expression of HSP70 and HSP90 in diaphragm. We conclude that exercise and whey protein consusumption can increase the activation of intracellular targets of protein synthesis during acute fasting protocol, these long-term adaptations could improve the maintenance of muscle tissue.