Cultivo in vitro de Schomburgkia crispa (Orchidaceae) com suplemento e meio alternativo à base de Chlorella sorokiniana (Chlorophyceae)

Abstract

The Schomburgkia crispa Lindlley (Orchidaceae), is an epiphyte, found in gallery forest and dry forests of the Cerrado, which, under natural conditions, presents difficulties of propagation. In order to evaluate the in vitro micropropagation of S. crispa, the present research was presented in two chapters. In chapter 1, the performance of the microalga Chlorella sorokiniana was evaluated as a supplement during the multiplication and in vitro rooting of S. crispa. In chapter 2, the performance of C. sorokiniana microalgae was evaluated as an alternative to the WPM commercial medium (Wood Plant Medium) during the in vitro micropropagation of S. crispa. Thus, in chapter 1, the culture medium and supernatant of C. sorokiniana microalgae were used at an average density of 107 x 105 mL-1 cells added to the WPM culture medium in place of distilled water. Different concentrations (0, 2.5 and 5.0 mg L-1) of 6-benzylaminopurine (BAP) and indolebutyric acid (AIB) were also added. In the in vitro multiplication of S. crispa the WPM culture medium with 5.0 mg L-1 of BAP increased the number of shoots while in in vitro rooting the WPM medium with 2.5 mg L-1 of IBA supplemented with cultivated medium of microalgae promoted an increase in the number of roots. In chapter 2, the culture medium and the supernatant of C. sorokiniana microalgae at an average density of 108 x 105 mL-1 cells were used as an alternative medium to the commercial WPM medium during the in vitro micropropagation of S. crispa. Explants of S. crispa cultivated in culture medium with the culture medium or the supernatant of C. sorokiniana microalga presented similar results to those cultured in WPM medium. In this way, it was possible to use the C. sorokiniana microalga as a supplement and an alternative medium to the WPM medium during the in vitro culture of S. crispa.