Contagem automática de micronúcleos em células de peixes

Abstract

The growth of urban areas and the population favored for increased pollution and contamination of river water. To be good indicators of pollution, the fish are widely used to assess water quality. Through the analysis of extracted animal blood cells, it is possible to identify pollutants and contaminants in rivers, through the micronucleus test. The micronucleus is a small body that arises during the process of cell division, and its presence is a strong indication of water contamination. The micronucleus count is a tedious and repetitive work susceptible to error. This paper presents a solution for automatic counting of micronuclei using the particle analyzer technique. In it are discussed two experiments, both using the software ImageJ library for the implementation of the solution. In the results obtained, the first experiment recognized the cells with micronuclei in 81.3% of cases, while the second recognized it in 88.3% of cases.