Atividade antioxidante e citotóxica do extrato hidroetanólico da casca de Nectandra cuspidata Nees & Mart. ex Nees

Abstract

Nectandra cuspitada is a plant popularly known in Brazil with laurel-pepper, laurel-cinnamon and is part of the native flora of the Brazilian cerrado. In this study, the antioxidant and cytotoxic activity of the hydroethanolic extract of the shell of Nectandra cuspidata (EHNC) was investigated. The content of phenolic compounds and flavonoids were determined using the Follin-Ciocalteau method and aluminum chloride, respectively. The antioxidant activity of the EHNC was evaluated by the free radical capture test 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). inhibition of oxidative hemolysis in erythrocytes induced by 2,2'-azobis amidinopropane (AAPH) followed by quantification of malondialdehyde (MDA) and investigation of the modulation of endogenous antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). Cell lines CCRF-CEM and Kasumi-1 were used to evaluate their cytotoxicity. The results show that the EHNC presents the content of phenolic compounds of 292.88 ± 11.49 mg EAG / g extract and Flavonoides 7.03 ± 1.18 mg EQ / g extract. In relation to its antioxidant capacity, it was able to inhibit 50% of the free radicals of DPPH and ABTS in 3.9 μg / mL and 1.99 μg / mL for the HNOS, and in 3.54 μg / mL and 3, 27 for ascorbic acid, respectively. EHNC was able to inhibit hemolysis and lipid peroxidation in human erythrocytes incubated with oxidizing agent, which was confirmed by MDA levels, being able to reduce by 66.39 ± 6.74%. EHNC was able to modulate the action of the CAT enzyme, increasing its activity at the lowest concentrations evaluated and no changes were observed in the enzymatic activity of SOD. The EHNC presented cytotoxic action against CCFR-CEM and Kasumi-1 cell lines. This study demonstrates for the first time that EHNC is capable of promoting antioxidant activity, modulates the endogenous activity of CAT, and promotes cytotoxic effects in leukemic cell lines, opening up new perspectives for the use of Nees as a natural safe product of Nectandra cuspidata and with antioxidant properties.