Bioprospecção de microalgas dulcícolas para ensaios ecotoxicológicos

Abstract

Microalgae has been the source of several studies in environmental applications given the ecological relevance and its autotrophic characteristic. The goal of the present work was to isolate and cultivate wild microalgae from canvas tanks of fish production so that they can be used in biotechnological processes. The techniques used for isolation were: collection by microcapillary, culture on agar, selection by aging of the culture medium and serial dilution. After their isolation, the microalgae was cultivated in NPK synthetic medium in the laboratory, in a non- axenic static system, with constant aeration, temperature of 25o C ± 1 and controlled photoperiod (12 h light / 12 h dark). The following microalgae were obtained: Chlorella sp, Closterium acerosum, Drepanochloris uherkovichii and Microcystis wesenbergii only two of the techniques used were efficient to isolation, the serial dilution and culture on agar.