Estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes)

Abstract

The mangabeira (Hancornia speciosa Gomes), belongs to the Apocynaceae Family being a fruitful tree found in several regions of Brazil. Its fruits present high nutritional value and are often used as raw material for the production of sweets, ice creams, juices and liqueurs, Its production, however, is almost entirely extractive, having few methods of commercial plantation, due to the difficulty of its natural propagation. The difficulty of mangabeira’s sexual propagation is mainly because of its seed’s recalcitrance, which means they don’t support storage in low temperatures and desiccation, and furthermore, the pulp of mangabeira’s fruits present germination inhibitors, that difficult its sexual propagation. An alternative to conter this propagation problem in the in vitro cultivation through micropropagation. This work had as its goal to review the capacity of in vitro establishment and germination of mangabeira explants, aiming the achievement of micropropagated seedlings. Therefore, three experiments were realized. Experiment 1: GA3’s effect on different concentrations (0; 2.5; 5.0 and 10 mg L1) on the in vitro germination. Experiment 2: Sodium hypochlorite’s effect on different immersion periods (5, 10 and 15 minutes) and on different concentrations (1.25 and 2.5%) on the in vitro establishment of nodal segments. Experiment 3: Effect of sodium hypochlorite (2.5%), PPM® (5%) and their association on the in vitro establishment of nodal segments. The explants’ germination was not observed, the main hindrance being the desiccation of the seeds and the fungal contamination that was of 100%. In the establishment using nodal segments, no established explants on any of the experiments were observed, being that the fungal contamination and the oxidation of the explants were the main cause of the establishment’s inefficiency, being the bacterial contamination extremely low on all treatments.