Seleção de inoculante bacteriano para produção de silagem de capim Piatã L. Plantarum e L. Brevis

Abstract

The objective of this experiment was to evaluate the microbiology and chemicals quality of grass Piatã (Brachiaria brizantha) silage enriched with different doses of crude glycerin and inoculated with new strains of lactic acid bactéria (LAB) selected to this forage. Was evaluated three strains of wild LAB which were previously isolated from the Piatã grass ensiling process and identified by 16S rDNA sequencing. Two of isolated strains of wild were identified as Lactobacillus plantarum and other as Lactobacillus brevis. Was made cut of grass, over a plastic canvas was chopped to decrease the particle size, heavy and mixed the inoculant with help of a spray bottle and deposited on PVC pipes (small silos), done compaction and sealing with plastic canvas and scotch tape, heavy and stored in the laboratory. After fermentation period the small silos was open and withdrawals sample for count microbiology and chemicals analyzes. The variables mineral matter, pH end and effluents loss presented statistical differences by Tukey test (5% probability). The remaining variables analyzed as crude protein (CP), dry matter (DM), mineral matter, pH initial, gases loss dry matter loss not presented statistical differences, but was presented some interactions, therefore are promising for use at tropical grass silage.