Adição de enzimas amilolíticas e celulolíticas em silagem de milho

Abstract

The objective of this study was to evaluate the interaction of different enzyme classes and effect on corn silage. 40 experimental silos were used in a 2x2 factorial arrangement, composing 4 treatments, with 10 silos per treatment. The treatments were: 1- Control (corn silage without addition of enzymes); 2-AMG (addition of 300 mL/t of amylolytic enzyme); 3- Celluloclast (addition of 300 mL/t of cellulolytic enzyme); 4-AMG + Celluloclast (150 mL/t amylolytic enzyme and 150 mL/t cellulolytic enzyme). The ensiled materials were inoculated with KERASIL (Lactobacillus plantarum: 4x1010 cfu/g + Pediococcus acidilactici: 4x1010 cfu/g) in the ratio of 4g/t. The corn silages plus CEL (Celluclast) showed higher losses per effluent and higher digestibility values in relation to the other treatments. In relation to pH, greater stability was observed for the CEL and GLU + CEL treatments in relation to the others. It was observed higher cellulose enzymatic activity for the CEL treatment and for amylase greater activity was obtained for GLU treatment, followed by GLU + CEL treatment for both cellulose and amylase. In the evaluation of lactic acid bacteria, lower counts were observed for GLU treatment compared to GLU + CEL, however, no differences were observed for CEL and CON treatments. The interaction between the glucoamylase and cellulose enzymes positively affected the nutritive value and the aerobic stability and negatively affected the fermentation and losses of corn silage.