Estudo in silico dos diferentes grupos de fosfolipase presentes no transcriptoma de Urochloa decumbens (Stapf) cv. Basilisk

Abstract

Urochloa decumbens (Stapf) cv. Basilisk (U. decumbens) is considered as an excellent forage, resistant to drought and grows in the most diverse types of soils; so it has great importance for the production of ruminants, since in Brazil the pastures represent the main and most economic resource for livestock. To further study the plant, its RNA was extracted by Embrapa Cattle from Campo Grande-MS, and sequenced by the Beying Genomics Institute in China. From this sequencing we found a set of genes coding for phospholipases, enzymes involved in plant signaling and immunity, and which are divided into phospholipase A1(PLA1), phospholipase A2(PLA2), phospholipase B(PLB), phospholipase C (PLC) and phospholipase D (PLD). PLB was the only non- Found in U. decumbens. From this sequencing Blastn and phylogenetic analyzes were performed to verify the similarity of these sequences with other phospholipases from different plants. This was an unpublished study and after analysis, it was observed that PLA1 and PLA2 have great similarity with millet (Setaria italica), PLA2 and PLD besides Setaria italica presented high similarity with braquiária (Brachypodium distachyon) and corn (Zea mays) respectively. The study of new cultivars, and the improvement of existing cultivars is and will always be necessary to maintain the quality of grazing areas in Brazil.