Avaliação da atividade antimicrobiana de nanopartícula de óxido de zinco (ZnO)

Abstract

Nanoparticles have generated great interest study for its unique physical and chemical characteristics provided by its reduced size and increased surface exposure. Some metal nanoparticles have been highlighted by having antimicrobial biological activity, even at low concentrations. The antimicrobial activity of metal zinc oxide (ZnO) nanoparticles has received special attention for its efficacy as the search for new agents in the control of resistant pathogens. The aim of this study was to synthesize ZnO nanoparticles by the method of co-precipitation and evaluate its antimicrobial potential of gram-negative and gram-positive in laboratory conditions. It was also made an analysis of FTIR spectrum, which confirmed the presence of Zn-O bands of nanoparticles. Nanoparticle dispersion was prepared in sterile water with Tween 80 so that it was determined the minimum inhibitory concentration (MIC). For this, an overnight bacterial growth was conducted with dilution in Mueller Hinton broth, increasing the count of 1×105 CFU/mL with the standardization of the strains. The MIC was established at 0.5 mg/ml for gram-positive bacteria and 1.0 mg/ml for gram-negative bacteria. These dispersion concentrations, it was not observed the presence of viable cells. This study demonstrates that the method of obtaining ZnO nanoparticles by co-precipitation was effective, and it has the potential to be incorporated into products and materials where it is crucial microbial control, besides the possibility of being used as alternative to conventional antibiotic drugs and with resistant microorganisms. Thus, it is essential to be made larger characterization and toxicity tests for future applications.