Potencial mutagênico e citotóxico de sementes de Aleurites moluccana (L.) Willd. in vitro

Abstract

Overweight is a public health problem and is associated with increased morbidity and mortality, this event encompasses issues of health and esthetic. Nowadays, the standards of beauty have changed, associating it with the slender body and slim, making people look for to lose weight to fit these models, through various means such as the use of natural products. However, these products may have undesirable side effects. In this sense, the objective was to assess the mutagenicity and cytotoxicity activity of Aleurites moluccana seed that has been popularly used for weight loss. The mutagenic potential was measured by the Ames test (Kado) with two strains of Salmonella Typhimurium TA 98 and TA 100 with and without metabolic activation and the cytotoxicity by MTS reduction assay with HeLa cells (human cervical adenocarcinoma). Was analyzed the number of revertants colonies per plate and mutagenicity index in the AMES test and seeds of A. moluccana demonstrated potential mutagenic at concentrations of 38.800 µg.mL-1 and 19.400 µg.mL-1 without metabolic activation for strain TA 98 and TA 100 at concentration 19.400 µg.mL-1 with and without metabolic activation. In the cytotoxicity assay, seeds of A. moluccana showed cytotoxic potential after 48 hours at concentrations of 38.800 µg.mL-1 and 19.400 µg.mL-1 indicating cell viability of 49.95% and 35.31% respectively. The results showed that seeds of A. moluccana exhibited mutagenic and cytotoxic potential in certain concentrations, showing risk of your consumption and also natural products in which its chemical composition and toxicity is unknown, however, do not know is what are the compounds that have given these activities seeds, requiring more testing and phytochemical analysis to ensure its use.