Estudo in silico dos diferentes grupos de expansina presentes no transcriptoma de Urochloa decumbens (Stapf) cv. Basilisk

Abstract

The Urochloa decumbens cv Basilisk is a forage grass that is very important in Brazilian cattle industry to be widely used in animal feed. It has advantageous features such as adaptation to various soil and climatic conditions, high nutritional quality and resistant to drought. Because of its importance and to deepen the knowledge about this plant, the plant RNA was extracted by EMBRAPA Gado e Corte (Campo Grande, MS) and sequenced by the Beijing Genomics Institute in China. An important set of genes which encode the expansin was found, proteins that are linked to the cell wall loosening, adjusting the expansion and growth of the plant. There was 88 expansins the transcriptome of Urochloa decumbens, which are divided into four groups, expansin type A, expansin type B, expansin A and expansin B. Of the 88 expansins, 38 are EXLA, 23 are EXLB, 8 are EXPA and 19 are EXPB. From these sequences were performed Blastn and phylogenetic analysis to verify the similarity and homology of these sequences with other expansins of different plants, and check the variations of them as time of evolution. From these analyzes, it was observed that the EXLA and EXLB groups have great similarity and homology with Setaria italica, noting that both sequences expansin EXLA and EXLB have a common ancestor with the Setaria italica, these results indicate that their sequences are more conserved. Unlike expansins groups EXPA and EXPB that showed greater similarity and homology with other plants such as Oryza sativa, Setaria italica, Phoenix dactylifera and Brassica rapa for EXPA and predominantly Zea mays for EXPB.