Atividade antimicrobiana, antioxidante, composição química, toxicidade e citotoxicidade das folhas de Cochlospermum regium

Abstract

The study of medicinal plants is important for the treatment of various diseases source, including fungal and bacterial infections. Cochlospermum regium (Cochlospermaceae) popularly known as algodãozinho-do-cerrado, is a plant widely used in medicine in Brazil. Among the various medicinal properties attributed to the plant, there is its use for the treatment diseases of infectious etiology and inflammation. The aim of this work was to evaluate the antimicrobial, antioxidant, key secondary metabolites and potential toxic and cytotoxic effects of the ethanol extract of the leaves of Cochlospermum regium. The antimicrobial activity was performed by the microdilution broth method. The antioxidant activity was determined with the DPPH free radical assay. To determine the chemical composition of the extract to high performance liquid chromatography was performed. Possible toxic and cytotoxic effects were evaluated by testing with Allium cepa. The ethanolic leaf extract showed activity against Candida krusei (64μg mL -1 ), Enterococcus faecalis (4μg mL-1 ) and Escherichia coli (8μg mL-1 ). The antioxidant activity showed an IC50 of 18,67 μg mL-1 . The phytochemical screening indicated the presence of flavonoids, phenols, tannins. The results showed that the tested concentrations did not cause toxicity, suggesting that the extract of C. regium does not interfere with cell division. This study demonstrated the antimicrobial and antioxidant activity of C. regium and absence of toxicity, indicating their potential for the development of new therapies for the treatment of infections.