Organogênese in vitro de Schomburgkia crispa Lindl. (Orchidaceae)

Abstract

The Schomburgkia crispa Lindl. is a epiphytic orchid species found Cerrado gallery forests and dry forests and considered an endangered species in the state of Minas Gerais. In recent decades the in vitro culture techniques is an important alternative to the rescue of endangered species and spread of several commercial species. This study aimed to evaluate the organogenic capacity using nodal segments and callus during in vitro multiplication of Schomburgkia crispa and the effect of different culture media, concentration and growth regulators. To achieve this purpose were conducted two separate experiments. In the first experiment was evaluated the multiplication capacity from nodal segments in different culture media (MS and WPM) and different concentrations (2.5, 5.0 and 7.5 mg L-1) of BAP (6- benzylaminopurine). In the second experiment was evaluated the multiplication capacity from callus grown on a medium supplemented with IAA (indole-3-acetic acid) and TDZ (thidiazuron), added to the culture medium combined in their concentrations 0; 0.1 + 0.1; 0.2 + 0.2; 0.3 + 0.3; 0.4 + 0.4 and 0.5 + 0.5 mg L-1 . In both experiments, the evaluations were performed after 30 days of in vitro culture, analyzed the number and length of shoots, leaf number, multiplication rate and additionally, in the second experiment, the callus diameter. In the in vitro multiplication of Schomburgkia crispa from nodal segments, growing in WPM medium supplemented with 5.0 mg L-1 BAP showed the best results. For multiplication from callus using the MS medium supplemented with 0.3 mg L-1 IAA and 0.3 mg L-1 TDZ induces more shoots.