Biomonitoramento dos efeitos genotóxicos de poluentes em tétrades de Tradescantia pallida (Rose) D.R Hunt. na cidade de Dourados-MS

Abstract

Purpose Relate the intensity of vehicle traffic in the city of Dourados (MS) with the number of micronuclei, stomatal index and size of the opening of the ostiole of the stomata in Tradescantia pallida. Methods From March to August 2010 was held collections of young inflorescences for analysis of Trad-MCN and analysis of leaf surface in four locations with different intensities of traffic vehicles. Statistical analysis was performed in SAS, using Tukey test at 5% for those who attended the assumptions, and Kruskal-Wallis 5% for those who did not attend, we also applied the Pearson simple correlation to calculate the 5%. Results When compared the occurrence of MCN between monitoring stations, each month, there was a significant difference (p <0.01) between them, may also see a significant difference in the interaction between data collection and point. The average rates and stomatal aperture size ostiole of the stomata in each sample point were not significantly different. We carried out frequency analysis of stomatal density of four points and can see that with increasing degree of pollution, the plants had an increase in stomatal density. Significant difference (p> 0.05) Kruskal-Wallis test between the points to the characteristic density of stomata. In the analysis of simple correlation between traits, there was only the negative association of -0.65 (p <0.05) between stomatal index and micronucleus. Conclusion The sites with higher vehicular traffic had significantly higher levels of contamination by genotoxic agents that can damage DNA and analysis of leaf surface showed physiological differences in density, content and opening their stomata.