Bioprospecção de bactérias em solo do cerrado com potencial de produção de polihidroxialcanoatos (PHA)

Abstract

Environmentally friendly alternatives to the use of conventional polymers have been studied with the purpose of reducing environmental impacts, such as polyhydroxyalkanoate (PHA) lipid biopolymers, biodegradable materials produced by bacteria and widely applied in the medical, pharmaceutical and chemical areas. Soils, in general, have a great diversity of organisms, with the possibility of biprospection of several species, thus, the objective of the present study was to bioprospect soil bacteria from the Cerrado biome, in order to evaluate the production potential of PHA biopolymer using glycerol agroindustrial residue as carbon source. in minimum culture medium ISP9. A total of 36 bacteria were isolated from the soil sample. An initial screening of lipid production by cells was performed by staining with Sudan Black, in which 20 of the bacteria tested positive. Taking into account the nonspecificity of this method, the staining technique was performed with Red Nile, fluorescent dye when exposed to ultraviolet light. In this evaluation 14 bacteria were identified as PHA producers. Thus, in the sampled soil it was possible to isolate biopolymer-producing bacteria, and glycerol proved to be a good alternative carbon source for the cultivation of these bacteria, which improves the economic viability of the process. To date no other studies involving the biopanning of PHA-producing bacteria from Cerrado soils have been described in the literature, showing the great biotechnological potential of this biome.