Caracterização de β-glicosidase produzida por cultivo em estado sólido do fungo Pycnoporus sanguineus

Abstract

Enzymes act as biocatalysts by increasing the speeds of some processes. These biocatalysts are used in various industrial processes, and it is possible to highlight the production of food, drugs, beverages, biofuels, paper and cellulose. Cellulases occupy about 20% of the enzyme market, and their efficiency in the hydrolysis of cellulosic material is the result of synergistic actions of an enzyme system composed of three enzymes which increases the cost of the process. The use of solid state cultivation (CES) makes it possible to reduce the costs of these biocatalysts due to the use of agroindustrial residues as substrate. The present work aimed to characterize the enzyme β-glycosidase produced by solid state cultivation of the fungus Pycnoporus sanguineus in wheat bran. The β-glycosidase showed optimal activity at pH 4.0 and 70°C. The enzyme remained stable at a pH range of 3.0 to 7.0 and maintained its activity (about 93,88% residual activity) after 1h at 50°C. The characteristics described for the enzyme are difficult to find for biocatalysts produced by mesophilic fungi, high stability at pH and temperature, which enables its use in industrial conditions.