Potencial antidiabético do extrato aquoso das folhas de Bidens gardneri Baker

Abstract

Bidens gardneri Baker has been traditionally used as a medicinal plant for the treatment of Diabetes mellitus. However, there is a lack of evidence on the benefits and mechanisms. This study aimed to evaluate the antidiabetic effect of B. gardneri (EABG) leaves aqueous extract. We evaluated the potential anti-glycation by the test (BSA) / glucose and after 14 days and 28 days after incubation were carried out the readings in a fluorimeter to determine the formation of Advanced Glycation End-products (AGEs). the content of phenols, flavonoids, flavonol and tannins were quantified. We identified the compounds by high performance liquid chromatography. The oral glucose tolerance test was performed in rats at different doses (30 mg / kg, 100 mg / kg and 300 mg / kg). Diabetic animals induced with alloxan received a daily dose of EABG (300 mg / kg V.O.) for 10 days. Blood glucose levels were used to assess their antidiabetic effects. Insulinresistant animals were induced with a hypercaloric diet for 14 weeks associated with treatment of daily dose of EABG (300 mg / kg V.O) over the 10-week period. After the animals were submitted to euthanasia, the activity of intestinal disaccharidases (maltase, sucrase and lactase) and quantification of the muscular and hepatic glycogen contents were determined. In the quantification of metabolites 4-O-caffeoylquinic acid was identified as the major compound. The extract presented potential of inhibition in the formation of advanced glycation products (AGEs). The study showed that EABG increased the concentration of hepatic and muscular glycogen and inhibited intestinal sucrase and lactase enzymes in normoglycemic animals. In the TOTG the dose of 300mg / kg of the EABG was the one that demonstrated the best effect in the decrease of the glycemia of the animals, besides having an effect similar to the drug glibenclamide. . In diabetic animals a daily dose of EABG given once daily for 10 days significantly lowered blood glucose levels, prevented weight loss, increased muscle glycogen content and inhibited the activity of intestinal enzymes. In addition, of the insulinresistant animals, the extract prevented the increase of glycemia, increasing the muscle glycogen content and inhibiting the activity of the intestinal enzymes. Our results suggest that the aqueous extract of Bidens gardneri presents potential for the treatment of diabetes.