Padronização do diagnóstico por amplificação simultânea das famílias alfaviridae e flaviviridae e diferencial da dengue, zika e chikungunya em soro de pacientes com suspeita clínica de doença arboviral

Abstract

Dengue is endemic in approximately 100 countries, affecting about 50 to 100 million people a year. In Brazil, it has been endemic since 1986 and the biggest epidemic happened in 2015, affecting over 1.5 million people. The Midwest region stands out as one of the regions with the highest incidence per 100,000 inhabitants. To contribute to molecular surveillance actions and containment planning, we analyzed blood samples from 121 patients with dengue-compliant clinical conditions in the Grande Dourados public and private health facilities, from July 30, 2016 to July 31, 2017. A structured questionnaire was applied to the candidates to participate in the research, containing socioeconomic and clinical information. Rapid tests for detection of anti-NS1 antibodies and RT techniques followed by PCR for etiological diagnosis. In this study it was possible to characterize the affected population and suggest a useful, fast and low value technique for the diagnosis of Dengue, Zika and Chikungunya. The data presented characterize the affected population and molecular techniques can be used in the laboratory routine as a tool to differentiate viruses from endemic regions, but it is still necessary to improve the reactions and primers used and to sequence the wild serotypes found.