Detecção do gene BLAoxa-58 em cepas de Acinetobacter baumannii isoladas de um hospital público de Dourados

Abstract

Acinetobacter baumannii is an opportunistic pathogen associated with a variety of infections, including pneumonias, urinary tract infections, and respiratory infections, often affecting immunocompromised patients. This organism has the ability to develop resistance to multiple antimicrobials, especially through the acquisition of genes that encode carbapenemases, such as oxacillinases OXA-23, OXA-24, and OXA-58, which, when expressed, confer resistance to carbapenems. In this study, the objective was to detect the gene encoding the oxacillinase OXA-58 in carbapenem-resistant A. baumannii strains. The analyzed samples were collected from Intensive Care Units (ICUs) of a public hospital in Dourados, MS. Bacterial identification and antibiotic susceptibility profiles were determined using the automated Phoenix® system. For the detection of the blaOXA-58 gene, the Polymerase Chain Reaction (PCR) technique was employed. The gene was detected for the first time in the state of Mato Grosso do Sul through the present work. With this, it is expected that this study will contribute to monitoring the circulation of this antimicrobial resistance gene in the region, deepening our understanding of its dissemination.