Bioprospecção de fungos filamentosos para produção de βglicosidase por cultivo em estado sólido

Abstract

The plant cell wall, composed of cellulose, hemicellulose and lignin is fundamental to the structure of plants. Cellulolytic enzymes, especially β-glucosidase are a crucial part in the degradation of lignocellulosic polymers, being used in the production of biofuels. This work aimed to isolate and select filamentous fungi from similar natural environments in different locations for the production of β-glucosidase in solid state cultivation using wheat bran as substrate. Twelve isolates were obtained and their β glucosidase production capabilities were evaluated. Isolates 2, 6, 9 and 12 demonstrated greater enzyme production, with isolate 9 being the most efficient with 36.75 U/g of dry substrate. Although the results obtained are lower than those described in the literature, they overcame the production of some previously studied species, indicating the potential of these isolates for future optimization and taxonomic identification studies. The report suggests that continued research could result in greater enzymatic yields and the exploration of other enzymes of industrial interest.