Anotação funcional de sequências genômicas de polihidroxialcanoato em Priestia megaterium cepa E1 isolada de solo

Abstract

Plastics are widely used due to their advantageous properties. However, its extensive use has caused several environmental problems mainly due to pollution and slow decomposition. Sustainable alternatives, such as bioplastics derived from renewable sources, are gaining interest. Examples of this are polyhydroxyalkanoates (PHAs) as viable alternatives to single-use plastics (SUPs), which are produced by microorganisms such as Priestia megaterium (formerly known as Bacillus megaterium). Genomics enters this scenario by playing an essential role in identifying genomes of microorganisms with potential for biosynthesis of PHAs for biotechnological application, especially through functional annotation programs. The present work intended to annotate genomic sequences of PHAs in the genome of the bacterium Priestia megaterium strain E1, isolated from soil samples collected in the region of Dourados, MS (2017). Functional annotation of genomic sequences of the bacterium in question was performed using the Prokka and eggNOG-mapper tools. The annotated sequences were subjected to local alignments in the Swiss-Prot (UniProt) and RefSeq_protein (NCBI) secondary databases. The results were also analyzed using the InteractiVenn software. Then, the sequences were subjected to global multiple alignments and phylogenetic analyses using ClustalX 2.1 and MEGA 11, respectively. A physicochemical analysis was performed using ProtParam software, and 3D modeling of PhaC synthase proteins was done using Phyre 2 and Swiss Model. Modeling of the other proteins was limited by the lack of data available in the literature. The results showed 25 PHA sequences, distributed among different enzymes such as PhaA, PhaB and PhaI, among others. The phylogenetic trees revealed homologies between the analyzed sequences and those taken from secondary databases, and the 3D modeling of the PhaC synthase proteins presented architectures compatible with previous studies. These results provided reliability to the annotations of PHAs in the genome of the bacterium Priestia megaterium strain E1, which corroborates scientific evidence already carried out on the species.