Eletrodo impresso de carbono em substrato de cera de abelha modificado com material carbonáceo@quitosana para a detecção de acetaminofeno

Abstract

In this work was studied the development of a sensible electrochemical platform, used as screen printed electrode on beewax substrate for Acetaminophen (AC) detection. The sensor was developed with graphite ink and varnish glass solubilized in acetone. The working electrode modification was made with a dispersion ofcarbon black (Vulcan XC72R) on chitosan. The chemical characterization was carried out by Fourier Transformed Infrared Spectroscopy. The sensors’ electrochemical characterization were made by cyclic voltammetry and chronoamperometry. These studies were conducted on support electrolyte 0,1 mol L -1 KCl with 1 mmol L -1 Fe(CN)6 3-/4- on a potential interval from -0,7 to +0,8 V vs graphite. AC electrochemical detection showed significant improvement in current response on the modified electrode by differential pulse voltammetry (DPV) at 0,1 mol L -1 phosphate buffer pH 6,5. Study on effect of pH on electrochemical behavior was carried out by DPV from 3,5 to 9,5 pH at 0,1 mol L -1 phosphate buffer. Concentration range studied was 0,050 to 4,5 µmol L -1 obtaining R 2 = 0,992 and LOD = 0,013 µmol L -1 . AC recovery study was conducted on real samples of river water, male urine and saliva, and a mean recovery rate of 103% were obtained, showing that the method of analyte detection presented high accuracy. The sensor demonstrated high stability along with reproducibility once current responses were not affected by beeswax substrate reutilization for new electrode’s production. This way, the electrochemical platform based on Vulcan@Chitosan is a low cost and easy application option for AC detection on different samples.