Efeito anti-inflamatório do extrato etanólico obtido da parte aérea do Blutaparon portulacoides

Abstract

Blutaparon portulacoides (capotiraguá) is found in the coastal regions of Brazil and is used ethnopharmacologically to treat leukorrhea and vulvovaginitis. Both diseases could indicate the presence of inflammatory and pathogen conditions. The aim of this study was the chemical characterization of ethanolic extracts derived from B. portulacoides (EEBP) stems and investigation to kill microorganism, as well as its effects against carrageenan or Freund's complete adjuvant (CFA) into the paws and on pleurisy induced with carrageenan or Mycobacterium bovis (BCG) of mice. The minimum inhibitory concentration of EEBP was determined for microorganisms, including Mycobacterium tuberculosis. In vivo experiments were undertaken to analyze the pleural exudates; one hour before, Swiss mice were orally administered EEBP, and carrageenan or BCG was injected into the lung pleural cavity. Paw inflammation induced by carrageenan or CFA was treated with EEBP. 3,5,3'-Trihydroxy-4'methoxy-6,7-methylenedioxy-flavone, gomphrenol, and ferulic, vanillic, and caffeic acids were identified using electrospray ionization–mass spectrometry and HPLC-MS/MS analysis. EEBP showed antibacterial activity against M. tuberculosis, S. typhimurium, and B. cepacia. EEBP has anti-inflammatory effects and is able to inhibit the increase in the IL-1β levels resulting from BCG- and carrageenan-induced pleurisy. EEBP reduced M. tuberculosis growth in vitro and ex vivo in organs shown by CFU assays. In the carrageenan-induced inflammation, EEBP was able to inhibit edema and mechanical hyperalgesia. Persistent EEBP treatment inhibited inflammation resulted from CFA-induced inflammation. The present study confirms the antiinflammatory and antibiotic properties of EEBP and these results may present new possibilities for the development of novel antibiotic drugs.