Interações biológicas entre Trissolcus urichi (Hymenoptera: Scelionidae) e Ooencyrtus submetallicus (Hymenoptera: Encyrtidae) em ovos de Nezara viridula e Chinavia pengue (Hemiptera: Pentatomidae)

Abstract

The study of the interaction between parasitoids and hosts is an important step for the multiplication process of parasitoids in laboratory conditions. The objective of this work was to evaluate the effects of the different ages of Ooencyrtus submetallicus (Hymenoptera: Encyrtidae) and Trissolcus urichi (Hymenoptera: Scelionidae) on their reproduction in Nezara viridula (Linnaeus , 1758) (Hemiptera: Pentatomidae); To verify the influence of age of N. viridula eggs on the biological performance of O. submetallicus and T. urichi; To verify the competition between O. submetallicus and T. urichi by N. viridula eggs and to analyze the potentiality of Chinavia pengue (Rolston, 1983) (Hemiptera: Pentatomidae) and N. viridula as alternative hosts of T. urichi under laboratory conditions. The experiments were conducted at the Laboratory of Biological Control of Insects (LECOBIOL) located in the Faculty of Biological and Environmental Sciences (FCBA), Federal University of Grande Dourados (UFGD), in Dourados, Mato Grosso do Sul. In all studies, the percentage of parasitism, the percentage of emergence, the duration of the life cycle (days), progeny (individuals per egg), sex ratio and longevity (days) were evaluated. In the first study, ages of 24, 48, 72, 96, 120 or 144 hours of O. submetallicus and T. urichi females were evaluated in 24-hour-old N. viridula eggs. The ages of 24, 48, 72, 96, 120 or 144 hours of N. viridula eggs were also evaluated for the creation of O. submetallcus and T. urichi. Females of O. submetallicus of all ages parasitized and reproduced in N. viridula eggs, of which 120 and 144 hours were the best ages for the creation of this parasitoid. T. urichi females of all ages parasitized, but did not develop into N. viridula eggs. O. submetallicus parasitized and developed on N. viridula eggs of all ages. T. urichi females parasitized, but only 7 individuals emerged. In the second study, the competition of 120-hour O. submetallicus females and 96-hour old T. urichi females was evaluated in N. viridula eggs. The parasitoid O. submetallicus was dominant in parasitizing and developing in N. viridula eggs. In the third study, the biological characteristics of T. urichi in N. viridula and C. pengue eggs were compared. Based on the methodology used and the results obtained, C. pengue is the best host for T. urichi parasitoid multiplication. Females of O. submetallicus are dominant on T. urichi females in the competition for N. viridula eggs and require up to 144 hours to mature their eggs. The stinkbug C. pengue is viable for alternative multiplication of T. urichi.