Análise de genes de resistência no transcriptoma de Panicum maximum Cv. tamani

Abstract

Species of the genus Panicum include important forages that stand out in Brazil, due to high productivity, adaptability to grazing, environmental conditions and management, especially in cultivated areas. This high adaptability is of great importance to the scientific community as it reveals genes that can be used for genetic transformation. Hydric stress can have significant impacts on plant growth and development. The genes that are expressed under these conditions can be analyzed through bioinformatics, which is a tool that enables different analyzes such as transcriptomics. With the present work, the objective was to search for resistance genes to P. maximum diseases through transcriptomy, raising the domains and classes of resistance proteins and doing 3D modeling of proteins with the domains found. This was done by performing a local alignment of the transcripts, organizing the results into a database. Next, a survey of domains and resistance classes was carried out, followed by 3D modeling. From the physiological condition of Panicum maximum water stress, the presence of 8 different resistance-related protein domains was verified, being the most expressive domains Serine-Threonine-Kinase, NBS and LRR. The expression of 10 different classes of resistance-related proteins was observed, the most expressive classes being RLP, NL and CNL; and similarity of resistance sequences between Panicum maximum and 13 different species was observed, being the species with more sequences similar Setaria italica, Panicum virgatum and Sorghum bicolor. The 3D modeling of proteins from each observed domain allowed a greater understanding of the functions of each of the modeled proteins, resulting in models with a high quality standard.