Análise metagenômica da rizosfera de Campomanesia adamantium (Cambess) O. Berg

Abstract

This work’s goal was to explore the biotechnological potential of the plant Campomanesia adamantium (Cambess) O. Berg by analyzing the bacteria and archaea community inhabiting its rhizosphere (zone under the influence of the plant roots). Knowledge of these bacterium taxonomy may lead to posterior formulating of a bioinoculante, a composition of bacterial cells that are able to promote plant growth through nitrogen-fixation, nutrient acquisition and competition. Besides, knowing these bacteria function could generate data for obtaining new microbial enzimes with biotechnological application potential. Therefore, the present work sought to identify the taxonomy and predict the functional of the microorganisms living in the rhizosphere of C. adamantium through next-generation sequencing and bioinformatics analysis. Soils were sampled from Horto Florestal (Orchard) at UFGD/Dourados – Mato Grosso do Sul, from which the genomic DNA was extracted. The DNA were sequenced in partnership with LMSeq – UNESP/Jaboticabal, by Ion Semiconductor’s Personal Genome Machine. The data was analyzed in two parameters of quality control and in two algorithmic methodologies due to possible discrepancies between bioinformatics analysis – the first one was BMP, Brazilian Microbiome Project and MICCA, Microbial Community Analysis. Lastly, the functional prediction was made through the PICRUSt package. Differences between the rhizosphere soil and no soil were found (q-value < 0,05), listing 15 bacteria and archaea genera who were superabundant in the rhizosphere region. Of these, 05 were associated in literature as nitrogen fixing and 03 associated with growth promotion by other means. 04 predicted proteins related to nitrogen fixation were found and 03 other proteins of biotechnological interest were abundant in C. adamantium’s rhizosphere. The multiple analysis of the same dataset generated statistically significant difference between each other. Hopefully, this new data could motivate the establishment of new guavirais and deepen it’s biotechnological exploration.