Investigação do potencial antimicrobiano e antioxidante, composição química, toxicidade e citotoxicidade da casca de canela vassoura (Ocotea minarum)

Abstract

Ocotea minarum (Lauraceae) popularly known as “canela vassoura”, is used in popular medicine to treat various diseases such as antirreumática and sudorífera properties. The objective was to investigate the antimicrobial activity by microbroth dilution assay to evaluate the antioxidant activity by DPPH test, identify secondary metabolites by liquid chromatography high efficiency and cytotoxic and toxic effects by Allium cepa test the ethanol bark extract's Ocotea minarum. The ethanol extract from the bark of O. minarum presented antimicrobial activity against Candida tropicalis ATCC 750 with an inhibitory effect of 256 mg L-1 , but when tested against clinical isolates of C. tropicalis, the extract did not present results of antimicrobial activity. The extract showed high antioxidant activity with IC50 25.94 mg L-1 . The phytochemical profile indicated the presence of phenol (31.29 mg g-1 ), flavonoids (16.52 mg g-1 ) and tannins (6.52 mg g-1 ) and two UV spectra peaks were similar to the spectrum strictosidinic acid, which is an indole alkaloid with analgesic and antirepáticas activities. There was no toxicity and cytotoxicity by Allium cepa technique. Thus, this study could be a starting point for further research for development of new antioxidants.