Quantificação e diversidade fenotípica de fungos isolados do solo com cultivo de soja (Glycine max (L.) Merrill) na região de Itaporã/MS

Abstract

This work had as main objective to quantitive and verify the different species of fungi found in soil cultivated with soybean (Glycine max). To this end, it was a demarcated area in which they withdrew, with the aid of an auger, three soil samples per collection. The collected soil was packed in plastic bags and led quickly to the laboratory for microbial analysis. The microbiota of active fungi in the form of colony forming units (UFC.ml-1), was evaluated by surface plating in key, in triplicate, and inoculation of serial suspensions using the medium of Martin (Martin 1950). Approximately 30 CFU / mL were found, each possible different species of fungus was transferred to a test tube and left at room temperature for them to grow in isolation and then were photographed digitally. Then it was done Microcultivation because it preserves the original layout of the spores on hyphae and keeps intact some spore-forming structures of each one, and finally the identification of genere fungi more abundance in the soil grown with Glycine max, among them we can highlight Aspergilus sp, Penicillium sp e Trichoderma sp. Finally done an analysis of variance, which showed a significant difference between the mean CFU before, during and after planting soybeans.