Caracterização molecular de Acinetobacter baumannii em um hospital público de Dourados/MS

Abstract

Acinetobacter baumannii is responsible to several cases of nosocomial infections. Once the increase in cases are related to nosocomial infections comprise high rates of morbidity and mortality, and to establish prolonged periods of hospitalization and high costs to the health system. The aim of the study was to evaluate and characterize strains of A. baumannii multidrug resistant in a Public Hospital of Dourados/MS. The bacterial strains of A. baumannii were collected from January to December 2014 and were identified by the automated system VITEK® (BioMérieux) and MALDI-TOF MS. During the study period were isolated 65 strains of A. baumannii from 65 ICU patients and attendance stations, from clinical samples, as rectal swab (21,5%), nasal swab (15,3%), tracheal aspirates (36,9%) secretion drainage (1,5%), biopsy material (1,5%), catheter (17,1%), ear secretion (1,5%), thigh secretion (1,5%), urine (7,6%), and blood cultures (6,1%).The evaluation of susceptibility profiles was determined by microdilution technique in broth, according to the CLSI wherein 96,9% of the strains were resistant to imipenem (MIC ≥ 8 mg / ml and 16 ug / ml) and 61 (93,8%) to meropenem (MIC ≥ 8 mg / mL and 16 ug / ml). The investigation of carbapenemases enzyme was determined by MALDI-TOF MS. The genetic analysis was performed by PCR to identify the following encoding genes of carbapenemases, blaKPC-2, blaIMP-1, blaVIM-1, blaNDM-1, blaOXA-23, blaOXA-24, blaOXA-51, blaOXA-48 e blaOXA-58. All strains were positive for blaOXA-51 gene and 90,7% for blaOXA-23 gene. Therefore identify the genes involved in bacterial resistance to carbapenems may assist in control measures hospitalar infection.