Propriedade antimicrobiana e atividade antioxidante do extrato etanólico das folhas de Smilax campestris Grisebach

Abstract

Medicinal plants represent an economical alternative, accessible and applicable to several diseases, especially in developing countries. Among them, people to treat flu, fevers, colds and diseases caused by microorganism commonly use the Smilax campestris Grisebach. In view of the growing search for new antimicrobial agents, increasing resistance of pathogenic microorganisms against the intensive use of synthetic products, this study aimed to evaluate the antimicrobial and antioxidant activity of the ethanol extract of the leaves of Smilax campestris Grisebach. The Minimum Inhibitory Concentration (MIC) was determined for 18 microorganisms of clinical interest through the broth microdilution technique and antioxidant test was carried out with free radical DPPH. Also quantitation of flavonoids, phenols and tannins been performed. The ethanolic extract of the leaves of Smilax campestris Grisebach showed activity for Staphylococcus epidermidis (MIC = 1000 ug/mL), and has shown good antioxidant activity with an IC50 of 13.1±0.1 ug/ml-1. Obtained the concentration of flavonoids of 70.3±1.6 mg EQ/g, phenols 123.9±1.0 mg GAE/g and condensed tannins 11.8±0.1 mg ACE/g. The results showed that the use of ethanol extract of the leaves of Smilax campestris Grisebach may be a promising alternative for the control of this pathogenic organism and as a great source of natural antioxidant.