Atividade antioxidante e teor de constituintes de Annona cacans, A. coriacea e A. sylvatica

Abstract

The antioxidant potential of extracts of the Annona cacans (pulp, seeds and leaves), A. coriacea and A. sylvatica (pulp and seeds) (Annonaceae) were investigated, as well as the total phenolics, flavonoids, condensed tannins and ascorbic acid total. The chemical composition was investigated, for the most active extract, using an analytical liquid chromatography (LC) method. Was used to determine the antioxidant activity the method of the 1,1-diphenyl-1-picrylhydrazyl free radical (DPPH), β-Carotene bleaching, and ABTS radical cations. The antioxidant activity of the seed extracts of A. cacans was the most active in free radical scavenging (IC50 of 25.6 μg/ml), β-carotene bleaching (AA% of 89.67 μg/ml), and the ABTS assay system (200.96 μM trolox/g sample). The seed extracts of A. cacans showed phenolic acid and flavonoids (luteolin and kaempferol) by LC; and also showed a high content of phenolic and condensed tannins. On comparative analysis between the extracts of A. coriacea and A. sylvatica, it was found that the seed extracts of A. coriacea demonstrated a moderate antioxidant effect and a free radical scavenging activity of 31.53%, by the DPPH test, 51.59% by the β-carotene bleaching test and 159.50 μM trolx/g of extract in the ABTS assay. We found that the hydromethanolic seed extract of A. coriacea had high total phenol (147.08 ± 4.20 mg of GAE/g of extract) and flavonoid (131.18 ± 2.31 mg of QE/g of extract) content. The antioxidant activity of the extracts was related to the contents of constituents present. These results indicate that A. cacans can be considered as a valuable source of antioxidant products when compared with the standard antioxidants.