Avaliação da atividade anti-inflamatória e antinociceptiva do extrato etanólico das folhas de Piper vicosanum em modelos experimentais

Abstract

Piper vicosanum is a plant belonging to the genus Piper, used in folk medicine as a diuretic, analgesic and anti-inflammatory. In the literature, there is a report of the antiinflammatory activity of the essential oil of Piper vicosanumleaves, however, there are still no studies evaluating the anti-inflammatory effect of the ethanolic extract of the leaves of Piper vicosanum. Thus, the objective of the present study was to evaluate the anti-inflammatory and antinociceptive effect of the ethanolic extract of the leaves of Piper vicosanum (EEPV) in experimental models in mice. For this, the EEPV was orally tested at doses ranging from 100 to 700 mg / kg in the pleurisy model and carrageenin-induced in situ mesenteric bed microcirculation. In addition, EEPV was orally tested at a dose of 700 mg / kg in zymosan-induced arthritis and peritonitis models. Nociceptive tests with intraplantar formalin injection and abdominal contortions induced by acetic acid were also performed, where the animals were treated with EEPV at doses of 300 and 700 mg / kg. EEPV wasable to inhibit the migration of leukocytes to the pleura induced by carrageenin in 26% (at the dose 700 mg / kg). Likewise, it reduced the bearing and adhesion of leukocytes in the in situ microcirculation model (at 300 mg / kg dose) in 48.48% and 48.20%, respectively. In zymosan-induced arthritis and peritonitis models, treatment with EEPV (at the dose of 700 mg / kg) reduced leukocyte recruitment by 40% and 41%, respectively. In the antinociceptive analysis, EEPV showed dose-dependent activity (doses 300 and 700 mg / kg) in the models of intraplantar injection of formalin and of abdominal writhings induced by acetic acid. Our results demonstrated for the first time the therapeutic potential of the ethanolic extract of Piper vicosanumleaves.