Produção de celulases e hemicelulases por fermentação em estado sólido do fungo Thermomyces lanuginosus em resíduos agroindustriais

Abstract

Many enzymes produced by fungi have significant industrial applications. The enzyme production by solid state fermentation reduces the production cost and decreases the environmental impact caused by such waste. The objective of this work was to study the production of cellulases and hemicellulases of Thermomyces lanuginosus fungus by solid state fermentation using different agro-industrial waste. We evaluated the solid substrates wheat bran, sugar cane bagasse, soybean meal, rice husk, corn straw and corncobs and then the fermentation time. The study showed that extracellular enzymes from the fungus T. lanuginosus is more than cellulolytic xylanolytic. The increased production of enzymes was obtained by the microorganism cultivation in wheat bran, containing 70% humidity, temperature 45 ° C, reaching about 21.1 ± 0.1 U / g dry substrate CMCase in 96 h of cultivation , 117.1 ± 2.5 U / g dry substrate β-glucosidase at 192 h 218.0 ± 5.8 U / g of xylanase dry substrate 144 h and 1.9 U / g dry substrate of β- xylosidase in 96 h of culture. The use of agricultural residues and optimization of fermentation parameters is important for future biotechnology applications of the enzyme production process.