Produção de enzimas fibrolíticas por cultivo em estado sólido de Pycnoporus sanguineus

Abstract

In Brazil, the high level of agricultural production generates a considerable amount of waste that can be utilized for solid-state cultivation to produce fibrolytic enzymes. The objective of this study was to assess the production of fibrolytic enzymes by the fungus Pycnoporus sanguineus utilizing agro-industrial waste as a substrate. The fungus P. sanguineus was cultivated in a solid state in a mixture of wheat bran and sugarcane bagasse, with the enzymes extracted and quantified at 24-hour intervals throughout the cultivation period. The highest enzyme production was observed at 48 and 72 hours for pectinase (1105.9 U.g-1) and β-glucosidase (8.30 U.g-1), and for endoglucanase (90.13 U.g-1) and xylanase (14.3 U.g-1) at 48 hours of cultivation. The fungus P. sanguineus was found to be an effective producer of fibrolytic enzymes, particularly pectinolytic enzymes, when cultivated in a solid-state using wheat bran and sugarcane bagasse as substrates.