Avaliação do efeito nefroprotetor do óleo de jacaré do Pantanal (Caiman yacare) em modelo experimental de rabdomiólise induzido por glicerol em ratos

Abstract

Acute kidney injury (AKI) is characterized by an abrupt loss of kidney function, with approximately 12–13 million cases annually, resulting in approximately 1.7 million deaths per year. The treatment of AKI represents a major cost burden for health systems, attributable to factors such as pharmacological treatment, severity of AKI, need for renal replacement therapy, and the lack of renal recovery. Rhabdomyolysis-induced AKI is one of the most frequently used experimental models, involving the administration of a single intramuscular injection of glycerol in rats. Therefore, this study aims to evaluate the nephroprotective effect of alligator oil (Caiman yacare) on glycerol-induced AKI in rats. The nephroprotective effects of alligator oil (OJ) were evaluated in a model of AKI (10 ml/kg glycerol, intramuscular) in Wistar rats. Different doses of OJ (19, 56 and 168 mg/kg) were administered orally for 7 days prior to AKI induction. Urinary parameters were measured during OJ treatment, biochemical parameters were assessed through blood collection were measured after OJ treatment. Electrocardiographic and hemodynamic parameters, renal vascular reactivity, and histopathological changes were measured after OJ treatment. The analyses revealed histopathological damage in the model used, reductions in 24-hour urinary excretion in the experimental groups, as well as alterations in electrocardiographic, biochemical and renal vascular reactivity parameters. However, treatment with alligator oil (Caiman yacare) did not influence any of the parameters analyzed, indicating that the intake of alligator oil during the study period did not demonstrate a nephroprotective effect against rhabdomyolysis-induced AKI in rats.